Quantitative detection of HIV-1 RNA using NucliSens EasyQ HIV-1 assay
文献类型：期刊论文
| 作者 | Yao, J; Liu, Z; Ko, LS; Pan, G; Jiang, Y |
| 刊名 | JOURNAL OF VIROLOGICAL METHODS
 |
| 出版日期 | 2005 |
| 卷号 | 129期号:1页码:40-46 |
| 关键词 | NucliSens EasyQ HIV-1 NucliSens HIV-1 QT NASBA HIV-1 RNA molecular beacons |
| 英文摘要 | HIV- 1 RNA viral load has become the major biological marker for disease prognosis and outcome of antiretroviral therapy in the treatment of HIV-infected individuals. The aim of this study was to compare the performance of the new CE marked NucliSens EasyQ HIV-1 assay with NucliSens HIV-1 QT assay (reference method). NucliSens EasyQ HIV-1 (EasyQ) couples nucleic acid sequence-based amplification (NASBA) with real-time detection using molecular beacons utilizing the NucliSens EasyQ analyzer. NASBA is a sensitive, isothermal, transcription-based amplification system designed specifically for the detection of RNA targets. There was significant correlation (r=0.878, P < 0.0001) between the two different assays in the analysis of clinical samples and the frequency of concordant results (log difference < 0.5) was 74%. The two assays detected HIV-1 RNA in 81 specimens, and neither detected (below the lower detection limit, 400 copies/ml for NucliSens HIV- I QT and 500 IU/ml for EasyQ) HIV- 1 RNA in 12 specimens. Three clinical specimens had detectable HIV- 1 RNA using the EasyQ only, and two specimens had detectable HIV- I=1 RNA using the NucliSens HIV- 1 QT only. The EasyQ procedure can analyze 48 clinical samples within 6 h. The coefficient of variation of EasyQ ranged from 3.0 to 9.5% (3% at 4.9 log, 5.7% at 3.7 log; 9.5% at 2.7 loa). The new assay is shown to be a rapid, convenient, and reliable procedure for HIV- 1 RNA viral load monitoring. (c) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS记录号 | WOS:000231936500005 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23260]  |
| 专题 | 生态环境研究中心_环境纳米材料实验室 |
| 推荐引用方式 GB/T 7714 | Yao, J,Liu, Z,Ko, LS,et al. Quantitative detection of HIV-1 RNA using NucliSens EasyQ HIV-1 assay[J]. JOURNAL OF VIROLOGICAL METHODS,2005,129(1):40-46. |
| APA | Yao, J,Liu, Z,Ko, LS,Pan, G,&Jiang, Y.(2005).Quantitative detection of HIV-1 RNA using NucliSens EasyQ HIV-1 assay.JOURNAL OF VIROLOGICAL METHODS,129(1),40-46. |
| MLA | Yao, J,et al."Quantitative detection of HIV-1 RNA using NucliSens EasyQ HIV-1 assay".JOURNAL OF VIROLOGICAL METHODS 129.1(2005):40-46. |
入库方式： OAI收割
来源：生态环境研究中心
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