Quantitative real-time RT-PCR for determination of vitellogenin mRNA in so-iuy mullet (Mugil soiuy)
文献类型：期刊论文
| 作者 | An, Lihui; Hu, Jianying; Zhang, Zhaobin; Yang, Min |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2006 |
| 卷号 | 386期号:2015-07-08页码:1995-2001 |
| 关键词 | biomarker endocrine-disrupting chemicals quantitative real-time reverse transcription polymerase chain reaction vitellogenin mRNA so-iuy mullet |
| 英文摘要 | A quantitative real-time reverse transcription polymerase chain reaction (Q- RT-PCR) assay was developed for quantification of vitellogenin (Vtg) mRNA normalized to beta-actin in so-iuy mullet. Vtg mRNA in liver samples of so-iuy mullet was induced after a single injection of E2 (0.01, 0.1, 1.0 mu g/g body) and a dose-response relationship was obtained. This method was applied to determine Vtg mRNA in so-iuy mullet collected from Liaodong Bay, Bohai Bay, NanDaiHe, and a control site in north China. Compared to the control, a high level of Vtg mRNA expression was detected in so-iuy mullets collected from NanDaiHe, whereas no obvious difference between Vtg mRNA expression from Liaodong Bay and Bohai Bay was found. Thus, this method is expected to be useful for further studying the potential of Vtg mRNA as a biomarker for assessing estrogenic activity in marine environments using the so-iuy mullet as a bioindicator species. |
| WOS记录号 | WOS:000242144200007 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23022]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | An, Lihui,Hu, Jianying,Zhang, Zhaobin,et al. Quantitative real-time RT-PCR for determination of vitellogenin mRNA in so-iuy mullet (Mugil soiuy)[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,386(2015-07-08):1995-2001. |
| APA | An, Lihui,Hu, Jianying,Zhang, Zhaobin,&Yang, Min.(2006).Quantitative real-time RT-PCR for determination of vitellogenin mRNA in so-iuy mullet (Mugil soiuy).ANALYTICAL AND BIOANALYTICAL CHEMISTRY,386(2015-07-08),1995-2001. |
| MLA | An, Lihui,et al."Quantitative real-time RT-PCR for determination of vitellogenin mRNA in so-iuy mullet (Mugil soiuy)".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 386.2015-07-08(2006):1995-2001. |
入库方式： OAI收割
来源：生态环境研究中心
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