A method to extract algae toxin of microcystin-LR
文献类型：期刊论文
| 作者 | Zhang, MM; Pan, G; Yan, H; Chen, H |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2004 |
| 卷号 | 16期号:4页码:694-696 |
| 关键词 | purification extraction microcystins HLB cartridge |
| 英文摘要 | A simple and low-cost method to obtain cyanobacterial toxin microcystin-LR(MC-LR) was developed. A new strain of Microcystis aeruginosa, named DC-1, producing microcystin-LR but not microcystin-RR, was separated from the field blooming algae samples of Dianchi Lake, in southwest of China. Following three times' freeze and thaw treatment, the cultivated DC-1 cells were extracted with 40% methanol in water. The extract was centrifuged and the supernatant applied to a Hydrophilic-Lipophilic Balance(HLB) SPE cartridge. Eluted impurities with a certain gradient from 30% to 50% methanol in water, MC-LR was finally eluted from the HLB cartridge with 60% methanol in water, and samples containing 3.85% to 14.8% of MC-LR were obtained. These MC-LR samples may be used in adsorption and biodegradation experiments instead of using expensive standard reagents. |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| WOS记录号 | WOS:000222450600036 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23502]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, MM,Pan, G,Yan, H,et al. A method to extract algae toxin of microcystin-LR[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2004,16(4):694-696. |
| APA | Zhang, MM,Pan, G,Yan, H,&Chen, H.(2004).A method to extract algae toxin of microcystin-LR.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,16(4),694-696. |
| MLA | Zhang, MM,et al."A method to extract algae toxin of microcystin-LR".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 16.4(2004):694-696. |
入库方式： OAI收割
来源：生态环境研究中心
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