Distribution of selenium-containing proteins in human serum
文献类型：期刊论文
| 作者 | Gao, YX; Liu, YB; Deng, GL; Wang, ZJ |
| 刊名 | BIOLOGICAL TRACE ELEMENT RESEARCH
 |
| 出版日期 | 2004 |
| 卷号 | 100期号:2页码:105-115 |
| 关键词 | selenium selenoprotein human serum |
| 英文摘要 | Selenium-containing proteins in human serum of four volunteers in Beijing were separated and purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Selenium contents in the proteins were quantified by high-performance liquid chromatography (HPLC) coupled with a fluorescence detector (FLD) after pretreatment with a microwave digestion system and derivatization with 2,3-diaminonaphthalene (DAN). Five selenium-containing proteins with apparent molecular weights (MWs) of 68+/-3, 57.5+/-2.5, 47+/-2, 41+/-1, and 21+/-1 kDa, respectively, were detected. By comparison with known data on serum selenium-containing proteins, the 68+/-3 kDa protein should belong to albumin, which took 6.3-9.8% of the total serum Se. The 57.5+/-2.5 kDa protein should be selenoprotein P and the 47+/-2 kDa protein was believed to be an isoform of selenoprotein P. The sum of Se in selenoprotein P and its isomer took about 41.1-69.3% and was the major form of human serum selenium. The 21+/-1 kDa protein should be plasma glutathione peroxidase (p-GPx) and its Se content was about 21.1-24.3%. Also, protein of 41+/-1 kDa should be a selenium-containing protein that, to our best knowledge, was reported for the first time. The Se percentage in this protein corresponded to 12.6-20.4% of total human serum Se. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| WOS记录号 | WOS:000223448900002 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23515]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Gao, YX,Liu, YB,Deng, GL,et al. Distribution of selenium-containing proteins in human serum[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2004,100(2):105-115. |
| APA | Gao, YX,Liu, YB,Deng, GL,&Wang, ZJ.(2004).Distribution of selenium-containing proteins in human serum.BIOLOGICAL TRACE ELEMENT RESEARCH,100(2),105-115. |
| MLA | Gao, YX,et al."Distribution of selenium-containing proteins in human serum".BIOLOGICAL TRACE ELEMENT RESEARCH 100.2(2004):105-115. |
入库方式： OAI收割
来源：生态环境研究中心
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