Activity-screening-guided isolation and purification for vasodilative effects compounds from Radix Astragali by high-speed counter-current chromatography using gradient elution
文献类型：期刊论文
| 作者 | Feng ZF(冯祖飞)1,2; Chen XF(陈小芬)1,2; Zhang, Jia3; Di DL(邸多隆)1,3 ; Di DL(邸多隆)
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| 刊名 | Natural Product Research
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| 出版日期 | 2013 |
| 卷号 | 27期号:11页码:1020-1022 |
| 关键词 | activity-screening-guided gradient elution high-speed counter-current chromatography Radix Astragali vasodilative effects |
| ISSN号 | 1478-6419 |
| 通讯作者 | 邸多隆 |
| 英文摘要 | The animal models were conducted to activity screening of different ethanol fraction from Radix Astragali. Eluted from the macroporous adsorptive resins, the potential active fraction that had vasodilative effects was isolated and purified by high-speed counter-current chromatography (HSCCC) using gradient elution. The solvent system was composed of chloroform-methanol-acetic acid-water (2:1:1:1, v/v/v/v). The flow rate was 1.0mLmin-1 initially and changed to 2.0mLmin-1 at 300min. Four flavonoids named calycosin-7-O--D-glycoside (a), formononetin-7-O--D-glycoside (b), calycosin (c) and formononetin (d) with purity over 94% were obtained and then identified by 1H NMR and 13C NMR. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the National Science and Technology Support Programme (No. 2007BA137B05);the National Natural Science Foundation of China (NSFC No. 21175142);the 'Hundred Talents Program' of CAS in 2007 |
| 语种 | 英语 |
| WOS记录号 | WOS:000320641900012 |
| 源URL | [http://210.77.64.217/handle/362003/18253]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Di DL(邸多隆) |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Gansu Coll Tradit Chinese Med, Dept Pharm, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng ZF,Chen XF,Zhang, Jia,et al. Activity-screening-guided isolation and purification for vasodilative effects compounds from Radix Astragali by high-speed counter-current chromatography using gradient elution[J]. Natural Product Research,2013,27(11):1020-1022. |
| APA | Feng ZF,Chen XF,Zhang, Jia,Di DL,&邸多隆.(2013).Activity-screening-guided isolation and purification for vasodilative effects compounds from Radix Astragali by high-speed counter-current chromatography using gradient elution.Natural Product Research,27(11),1020-1022. |
| MLA | Feng ZF,et al."Activity-screening-guided isolation and purification for vasodilative effects compounds from Radix Astragali by high-speed counter-current chromatography using gradient elution".Natural Product Research 27.11(2013):1020-1022. |
入库方式： OAI收割
来源：兰州化学物理研究所
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