Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application
文献类型：期刊论文
| 作者 | Liu, Yewei1; Bu, Lingna1; Zhao, Jianxi1; Wei JT(魏鉴腾)2,3
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| 刊名 | Journal of Liquid Chromatography and Related Technologies
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| 出版日期 | 2015 |
| 卷号 | 38期号:12页码:1179-1184 |
| ISSN号 | 1082-6076 |
| 关键词 | metabolomics antioxidant capacity intracellular metabolite evaluation approach biomarker |
| 通讯作者 | Liu, Yewei |
| 英文摘要 | Metabolomics is frequently used in the analysis of the dynamic changes within small molecules in response to the disturbance in an organism. It has been extensively used in various fields involving human diseases. To better evaluate the antioxidant capacity, cells subjected to oxidative stress were cultured, and the intracellular metabolites were prepared using HSDE (high-speed shear dispersing emulsifier)-assisted extraction. The metabolites were then analyzed under optimized chromatographic conditions. Partial least squares discriminate analysis was used to analyze the data. A new intracellular metabolomic approach was established, and four potential metabolic biomarkers for repairing oxidative stress were found. The antioxidant activities of Cynomorium songaricum was successfully evaluated using the intracellular metabolomic approach, indicating the feasibility of the proposed approach as an effective tool for evaluating the antioxidant capacity. |
| 学科主题 | 分析化学与药物化学 |
| 资助信息 | the National Natural Science Foundation of China (20974116;21175142);National High Technology Research and Development Program of China (2014AA022203);Science Foundation of Gansu Province (1308RJYA077) |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355595800002 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://210.77.64.217/handle/362003/18469]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 作者单位 | 1.Lanzhou Univ, Inst Nutr & Food Hyg, Sch Publ Hlth, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources and Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Ctr Resource Chem & New Mat, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yewei,Bu, Lingna,Zhao, Jianxi,et al. Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application[J]. Journal of Liquid Chromatography and Related Technologies,2015,38(12):1179-1184. |
| APA | Liu, Yewei,Bu, Lingna,Zhao, Jianxi,&Wei JT.(2015).Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application.Journal of Liquid Chromatography and Related Technologies,38(12),1179-1184. |
| MLA | Liu, Yewei,et al."Intracellular Metabolomic Approach for Evaluating Antioxidant Capacity and Its Application".Journal of Liquid Chromatography and Related Technologies 38.12(2015):1179-1184. |
入库方式： OAI收割
来源：兰州化学物理研究所
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