A BODIPY-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in solution and in cells
文献类型：期刊论文
| 作者 | Wang JM(王佳敏)1,2,3 ; Yu H(于辉)1,2,3; Li Q(李欠)1,2; Shao SJ(邵士俊)1,2
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| 刊名 | Talanta
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| 出版日期 | 2015 |
| 卷号 | 144页码:763-768 |
| 关键词 | Fluorescent probe BODIPY 7-Nitrobenzoxadiazole (NBD) H2S Bioimaging |
| ISSN号 | 0039-9140 |
| 通讯作者 | 邵士俊 |
| 英文摘要 | A new turn-on fluorescent probe, incorporating 7-nitrobenzoxadiazole (NBD) ether group into a BODIPY molecule, was synthesized and studied for the detection of H2S and biothiols in aqueous solution and in living cells. The design was based on thiol-induced thiolysis of the NBD ether bond and followed by the cleavage and the release of free meso-(4-hydroxybenzyI)-substituted BODIPY fluorophore. The BODIPY-based probe displayed highly sensitive and selective fluorescence enhancement respond to H2S over competing biothiols such as cysteine (Cys) and glutathione (GSH), mainly due to the weak reactivity of biothiols toward the probe. There was a good linearity between the fluorescence intensity and the concentrations of H2S in the range of 1-200 mu M with a detection limit of 2.6 mu M. The proposed reaction mechanism was confirmed by mass spectrometry and optical spectroscopy, and the mechanism of turn-on fluorescent response was further determinated by the density functional theory (DFT) calculations using Gaussian 03 program. Moreover, the probe was successfully applied for the fluorescence imaging of H2S in HeLa cells under physiological conditions (pH 7.4). (C) 2015 Elsevier B.V. All rights reserved. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the National Natural Science Foundation of China, China (20972170;21275150);the Funds for Distinguished Young Scientists of Gansu (1210RJDA013) |
| 语种 | 英语 |
| WOS记录号 | WOS:000363346200105 |
| 源URL | [http://210.77.64.217/handle/362003/18765]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang JM,Yu H,Li Q,et al. A BODIPY-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in solution and in cells[J]. Talanta,2015,144:763-768. |
| APA | Wang JM,Yu H,Li Q,&Shao SJ.(2015).A BODIPY-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in solution and in cells.Talanta,144,763-768. |
| MLA | Wang JM,et al."A BODIPY-based turn-on fluorescent probe for the selective detection of hydrogen sulfide in solution and in cells".Talanta 144(2015):763-768. |
入库方式： OAI收割
来源：兰州化学物理研究所
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