Quantitative analysis of five toxic alkaloids in Aconitum pendulum using ultra-performance convergence chromatography (UPC2) coupled with mass spectrometry
文献类型：期刊论文
| 作者 | Zhao TJ(赵堂娟)1,2,3; Qi HY(戚欢阳)1,2 ; Chen J(陈娟)1,2; Shi YP(师彦平)1,2; Shi YP(师彦平); Chen J(陈娟)
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| 刊名 | RSC Advances
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| 出版日期 | 2015 |
| 卷号 | 5期号:126页码:103869-103875 |
| ISSN号 | 2046-2069 |
| 通讯作者 | 陈娟 ; 师彦平 |
| 英文摘要 | A rapid and efficient ultra-performance convergence chromatography (UPC2) method coupled with electrospray ionization single quadrupole mass spectrometry (ESI-MS) was developed and validated for the simultaneous quantification of five diester diterpenoid alkaloid constituents (3-acetylaconitine, hypaconitine, deoxyaconitine, mesaconitine, aconitine) in Aconitum pendulum. Optimum separation was achieved on a BEH 2-EP C18 column (2.1 x 150 mm i.d., 1.7 μm particle) with a gradient elution of a mixture of A (supercritical CO2) and B (methanol containing 10 mmol L-1 ammonium acetate) and at a flow rate of 0.8 mL min-1 within 3 minutes. Quantification was performed using mass spectrometry in a positive ion ionization mode and selected ion recording (SIR) mode. The influences of column, modifier, additive, column temperature, and back pressure were investigated. The five alkaloids were identified and quantified using a comparison of retention time, ultraviolet spectrum, molecular ion peak (obtained from a selective ion recording mode) and peak areas with the reference compounds. The method was validated through linearity, limits of detection, limits of quantification, precision, stability, repeatability, and accuracy. The validated method was applied to analyze A. pendulum, which provided a reference for the quality evaluation of A. pendulum. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the National Natural Science Foundation of China (No. 21375136;21575150);the Scholar Program of West Light Project, Chinese Academy of Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000366433700015 |
| 源URL | [http://210.77.64.217/handle/362003/18974]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Shi YP(师彦平); Chen J(陈娟) |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao TJ,Qi HY,Chen J,et al. Quantitative analysis of five toxic alkaloids in Aconitum pendulum using ultra-performance convergence chromatography (UPC2) coupled with mass spectrometry[J]. RSC Advances,2015,5(126):103869-103875. |
| APA | Zhao TJ,Qi HY,Chen J,Shi YP,师彦平,&陈娟.(2015).Quantitative analysis of five toxic alkaloids in Aconitum pendulum using ultra-performance convergence chromatography (UPC2) coupled with mass spectrometry.RSC Advances,5(126),103869-103875. |
| MLA | Zhao TJ,et al."Quantitative analysis of five toxic alkaloids in Aconitum pendulum using ultra-performance convergence chromatography (UPC2) coupled with mass spectrometry".RSC Advances 5.126(2015):103869-103875. |
入库方式： OAI收割
来源：兰州化学物理研究所
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