Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry
文献类型：期刊论文
| 作者 | Sun YP(孙英佩)1,2,3; Chen J(陈娟)1,2 ; Qi HY(戚欢阳)1,2; Shi YP(师彦平)1,2; Qi HY(戚欢阳); Shi YP(师彦平)
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| 刊名 | Journal of Chromatography B
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| 出版日期 | 2015 |
| 卷号 | 1004页码:53-59 |
| 关键词 | Uric acid (UA) Graphitic carbon nitrides (g-CNs) Hollow fiber (HF) Solid phase microextraction (SPME) Gas chromatography-mass spectrometry (GC/MS) |
| ISSN号 | 1570-0232 |
| 通讯作者 | 戚欢阳 ; 师彦平 |
| 英文摘要 | An elevated uric acid (UA) in urine or serum can affect renal function and blood pressure, which is an indicator of gout, cardiovascular and renal diseases, hypertension, etc. In this work, a new type of mixed matrix membrane (MMM), based on graphitic carbon nitrides (g-CNs) and hollow fiber (HF), was prepared and combined with solid phase microextraction (SPME) mode to determine UA in urine and serum followed by gas chromatography-mass spectrometry (GC/MS). The porous g-CNs were dispersed in ammonia, and then the exfoliated g-CNs nanosheets were held in the pores of HF by capillary forces and sonification. The prepared g-CNs modified HF (g-CNs-HF) was immersed in biofluid directly to extract UA with SPME mode and the solvent-free mode is convenient for further derivatization and analysis. To achieve the highest extraction efficiency (EF), main extraction and derivatization parameters, such as g-CNs-HF immobilizing time, sonification power and time of extraction, derivatization and desorption time, were optimized. Under the optimum extraction conditions, a favorable linearity of UA was obtained in the range 0.1-200 mu g mL(-1) with correlation coefficients higher than 0.9990, and the average recoveries at three spiked levels of UA in urine and serum ranged from 80.7% to 121.6%, from 84.7% to 101.1%, respectively. The obtained results demonstrated the developed g-CNs-HF-SPME is a simple, rapid, cost-effective, solvent-free method for the analysis of UA in biofluid. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the National Nature Science Foundation of China (Nos. 21375136;21105106);the Scientific research project of Central Asia Drug Discovery and Development Centre of Chinese Academy of Sciences (No. CAM201404) |
| 语种 | 英语 |
| WOS记录号 | WOS:000364246300008 |
| 源URL | [http://210.77.64.217/handle/362003/18978]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Qi HY(戚欢阳); Shi YP(师彦平) |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun YP,Chen J,Qi HY,et al. Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry[J]. Journal of Chromatography B,2015,1004:53-59. |
| APA | Sun YP,Chen J,Qi HY,Shi YP,戚欢阳,&师彦平.(2015).Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry.Journal of Chromatography B,1004,53-59. |
| MLA | Sun YP,et al."Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry".Journal of Chromatography B 1004(2015):53-59. |
入库方式： OAI收割
来源：兰州化学物理研究所
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