Fingerprint analysis of Oxytropis falcate using ultraperformance liquid chromatography–electrospray ionization tandem mass spectrometry(UPLC-ESIMS)
文献类型：期刊论文
| 作者 | Zhao TJ(赵堂娟)1,2 ; Chen J(陈娟)1; Shi YP(师彦平)1; Shi YP(师彦平); Chen J(陈娟)
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| 刊名 | Analytical Methods
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| 出版日期 | 2015 |
| 卷号 | 7页码:6810-6820 |
| ISSN号 | 1759-9660 |
| 通讯作者 | 陈娟 ; 师彦平 |
| 英文摘要 | An efficient, accurate and reliable chromatographic fingerprint based on ultra-performance liquid chromatography coupled with photodiode array detection and electrospray ionization mass spectrometry (UPLC-PDA-ESI-MS) was developed for the identification and quality control of Oxytropis falcate Bunge. The method was validated for precision, repeatability, stability, and specificity. The RSD\ values of retention times and peak areas of the common peaks were 0–0.7% and 1.0–3.6% for withinday precision, while 0–0.5% and 1.5–6.1% for between-day precision. For repeatability, the values were lower than 0.5% and 2.6%, respectively, and for stability (24 h), the values were lower than 0.9% and 3.3%, respectively. Twenty-one batches of O. falcate obtained from different regions were analyzed by employing the developed method. Several multivariate statistical analysis including similarity analysis (SA), hierarchical clustering analysis (HCA) and principal component analysis (PCA) were applied to cluster and evaluate the O. falcate samples according to their collection sites. The results showed that the quality of O. falcate did not merely depend on the geographic origin. Thirty common peaks were found in the chromatographic fingerprints of O. falcate and eleven of them were identified by comparing the retention time, the ultraviolet spectrum and the molecular ion peaks (obtained from selective ion recording mode) with the reference compounds. This method with high precision, reliable stability an dgood repeatability can provide a reference for the quality evaluation of O. falcate. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the National Natural Science Foundation of China (Nos 21105106;21375136);the Scholar Program of West Light Project, Chinese Academy of Sciences |
| 语种 | 英语 |
| 源URL | [http://210.77.64.217/handle/362003/18979]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Shi YP(师彦平); Chen J(陈娟) |
| 作者单位 | 1.Key Laboratory of Chemistry of Northwestern Plant Resources of CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China 2.University of Chinese Academy of Sciences, Beijing 100039, P. R. China |
| 推荐引用方式 GB/T 7714 | Zhao TJ,Chen J,Shi YP,et al. Fingerprint analysis of Oxytropis falcate using ultraperformance liquid chromatography–electrospray ionization tandem mass spectrometry(UPLC-ESIMS)[J]. Analytical Methods,2015,7:6810-6820. |
| APA | Zhao TJ,Chen J,Shi YP,师彦平,&陈娟.(2015).Fingerprint analysis of Oxytropis falcate using ultraperformance liquid chromatography–electrospray ionization tandem mass spectrometry(UPLC-ESIMS).Analytical Methods,7,6810-6820. |
| MLA | Zhao TJ,et al."Fingerprint analysis of Oxytropis falcate using ultraperformance liquid chromatography–electrospray ionization tandem mass spectrometry(UPLC-ESIMS)".Analytical Methods 7(2015):6810-6820. |
入库方式： OAI收割
来源：兰州化学物理研究所
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