A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction
文献类型：期刊论文
| 作者 | Tian, Y; Wang, Y; Xu, Y; Liu, Y; Li, D; Fan, CH |
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2015 |
| 卷号 | 58期号:3页码:514—518 |
| 关键词 | CDSE/ZNS QUANTUM DOTS GOLD NANOPARTICLES DNA DETECTION METAL-IONS LABEL-FREE HG2+ IONS DNAZYME DUPLEXES WATER CU2+ |
| 英文摘要 | In this paper, we report a highly sensitive chemiluminescence (CL) sensor for Hg2+ ions based on thymine-Hg2+-thymine (T-Hg2+-T) coordination chemistry. We designed a thymine rich oligonucleotide as a capture probe and a signal probe that includes two functional domains: a horseradish peroxidase-mimicking DNAzyme domain for the generation of CL, and a recognition domain. Graphene oxide (GO) was introduced to adsorb the signal probe via p-p interaction, which brought the DNAzyme domain and GO into close proximity and quenches CL. In the presence of Hg2+ ions, the coordination of Hg2+ with the capture probe yielded a hairpin complex, triggers cascaded strand displacement reactions and Exonuclease III-assisted signal amplifications. As a result, accumulated amounts of DNAzyme were generated and released from GO, leading to an enhanced CL signal. This strategy combines enzyme-based signal amplification and GO as a background reducer, leads to a limit of detection (LOD) of 2 nmol/L. This simple detection system provides a label-free yet sensitive approach for detection of Hg2+ ions. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24370]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Tian, Y,Wang, Y,Xu, Y,et al. A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction[J]. SCIENCE CHINA-CHEMISTRY,2015,58(3):514—518. |
| APA | Tian, Y,Wang, Y,Xu, Y,Liu, Y,Li, D,&Fan, CH.(2015).A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction.SCIENCE CHINA-CHEMISTRY,58(3),514—518. |
| MLA | Tian, Y,et al."A highly sensitive chemiluminescence sensor for detecting mercury (II) ions: a combination of Exonuclease III-aided signal amplification and graphene oxide-assisted background reduction".SCIENCE CHINA-CHEMISTRY 58.3(2015):514—518. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。