A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting
文献类型：期刊论文
| 作者 | Wang, SM; He, X; Li, N; Yu, F; Hu, Y; Wang, LS; Zhang, P; Du, YK; Du, SS; Yin, ZF |
| 刊名 | INTERNATIONAL JOURNAL OF NANOMEDICINE
 |
| 出版日期 | 2015 |
| 卷号 | 10页码:2857—2869 |
| 关键词 | INHALED CORTICOSTEROIDS ANTIBODY FRAGMENTS ALBUMIN NANOPARTICLES CANCER-CELLS TUMOR-CELLS DELIVERY THERAPY GROWTH INHIBITION EXPRESSION |
| 英文摘要 | Lung-targeting drugs are thought to be potential therapies of refractory lung diseases by maximizing local drug concentrations in the lung to avoid systemic circulation. However, a major limitation in developing lung-targeted drugs is the acquirement of lung-specific ligands. Pulmonary surfactant protein A (SPA) is predominantly synthesized by type II alveolar epithelial cells, and may serve as a potential lung-targeting ligand. Here, we generated recombinant rat pulmonary SPA (rSPA) as an antigen and immunized an alpaca to produce two nanobodies (the smallest naturally occurring antibodies) specific for rSPA, designated Nb6 and Nb17. To assess these nanobodies' potential for lung targeting, we evaluated their specificity to lung tissue and toxicity in mice. Using immunohistochemistry, we demonstrated that these anti-rSPA nanobodies selectively bound to rat lungs with high affinity. Furthermore, we intravenously injected fluorescein isothiocyanate-Nb17 in nude mice and observed its preferential accumulation in the lung to other tissues, suggesting high affinity of the nanobody for the lung. Studying acute and chronic toxicity of Nb17 revealed its safety in rats without causing apparent histological alterations. Collectively, we have generated and characterized lung-specific nanobodies, which may be applicable for lung drug delivery. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24380]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wang, SM,He, X,Li, N,et al. A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2015,10:2857—2869. |
| APA | Wang, SM.,He, X.,Li, N.,Yu, F.,Hu, Y.,...&Li, HP.(2015).A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting.INTERNATIONAL JOURNAL OF NANOMEDICINE,10,2857—2869. |
| MLA | Wang, SM,et al."A novel nanobody specific for respiratory surfactant protein A has potential for lung targeting".INTERNATIONAL JOURNAL OF NANOMEDICINE 10(2015):2857—2869. |
入库方式： OAI收割
来源：上海应用物理研究所
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