Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles
文献类型：期刊论文
| 作者 | Wang, JJ; Lu, JX; Su, S; Gao, JM; Huang, Q; Wang, LH; Huang, W; Zuo, XL |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2015 |
| 卷号 | 65页码:171—175 |
| 关键词 | SMALL MOLECULES COLORIMETRIC DETECTION APTAMER APTASENSOR SIGNAL PROBE ASSAY AMPLIFICATION DIAGNOSTICS SEQUENCES |
| 英文摘要 | The detection of small molecules depends heavily on complicated GC-MS (Gas chromatography-mass spectrometry), HPLC (High-performance liquid chromatography) and some other complicated instruments that are not suitable for point of care detection. Here, we have demonstrated a fast (in 10 min), simple (instrument-free) and effective detection platform for small molecule-ATP. In our design, we engineered the hybridization region of aptamer and assembled it into a superstructure to avoid the exposed flexible ends. The binding of ATP triggered the collapse of the superstructures to produce single stranded DNA that can obviously tune the plasmonic coupling of unmodified gold nanoparticles (AuNPs). Compared to detection platforms based on fully hybridized aptamer double helix, the detection time was significantly decreased to 10 min. The resulting color change can be recognized by naked eyes. Our detection is highly specific and selective. Furthermore, a logic gate with multiplexed detection capability for ATP and DNA were demonstrated. (C) 2014 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24400]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wang, JJ,Lu, JX,Su, S,et al. Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles[J]. BIOSENSORS & BIOELECTRONICS,2015,65:171—175. |
| APA | Wang, JJ.,Lu, JX.,Su, S.,Gao, JM.,Huang, Q.,...&Zuo, XL.(2015).Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles.BIOSENSORS & BIOELECTRONICS,65,171—175. |
| MLA | Wang, JJ,et al."Binding-induced collapse of DNA nano-assembly for naked-eye detection of ATP with plasmonic gold nanoparticles".BIOSENSORS & BIOELECTRONICS 65(2015):171—175. |
入库方式： OAI收割
来源：上海应用物理研究所
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