Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway
文献类型：期刊论文
| 作者 | Ran, TT; Gao, MX; Wei, QE; He, JH; Tang, L; Wang, WW; Xu, DQ |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
 |
| 出版日期 | 2015 |
| 卷号 | 71页码:149—152 |
| 关键词 | CHROMOBACTERIUM-VIOLACEUM JANTHINOBACTERIUM-LIVIDUM HETEROLOGOUS EXPRESSION GENE-CLUSTER REARRANGEMENT TRYPANOCIDE TRYPTOPHAN MOLECULE CLONING |
| 英文摘要 | Violacein, a natural purple secondary metabolite, is sequentially biosynthesized by five enzymes in the following pathway: VioA-VioB-VioE-VioD-VioC. VioD, a flavin-dependent oxygenase, catalyzes the hydroxylation of the intermediate product prodeoxyviolaceinic acid (PVA) at the 5-position of one indole ring to yield proviolacein. In vitro biochemical data have revealed this process, but the catalytic mechanism still remains largely unclear. Here, the cloning, expression, purification, crystallization and diffraction of VioD are reported. Crystals of VioD diffracted to 1.7 angstrom resolution and belonged to space group P3(1), with unit-cell parameters a = b = 90.0, c = 94.5 angstrom, alpha = beta = 90, gamma = 120 degrees. Solvent-content calculation and molecular-replacement results suggest the presence of two molecules of VioD in the asymmetric unit. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24459]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Ran, TT,Gao, MX,Wei, QE,et al. Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2015,71:149—152. |
| APA | Ran, TT.,Gao, MX.,Wei, QE.,He, JH.,Tang, L.,...&Xu, DQ.(2015).Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,71,149—152. |
| MLA | Ran, TT,et al."Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 71(2015):149—152. |
入库方式： OAI收割
来源：上海应用物理研究所
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