Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific gamma-Glutamyltranspeptidase
文献类型：期刊论文
| 作者 | Wang, FY; Zhu, Y; Zhou, L; Pan, L; Cui, ZF; Fei, Q; Luo, SH; Pan, D; Huang, Q; Wang, R |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2015 |
| 卷号 | 54期号:25页码:7349—7353 |
| 关键词 | PROTEASE ACTIVITY FLUOROGENIC PROBE TURN-ON CELLS DESIGN GLUTATHIONE CYSTEINE TRANSFERASE GLUTAMYLTRANSFERASE HOMOCYSTEINE |
| 英文摘要 | gamma-Glutamyltranspeptidase (GGT) is a tumor biomarker that selectively catalyzes the cleavage of glutamate overexpressed on the plasma membrane of tumor cells. Here, we developed two novel fluorescent in situ targeting (FIST) probes that specifically target GGT in tumor cells, which comprise 1) a GGT-specific substrate unit (GSH), and 2) a boron-dipyrromethene (BODIPY) moiety for fluorescent signalling. In the presence of GGT, sulfur-substituted BODIPY was converted to amino-substituted BODIPY, resulting in dramatic fluorescence variations. By exploiting this enzyme-triggered photophysical property, we employed these FIST probes to monitor the GGT activity in living cells, which showed remarkable differentiation between ovarian cancer cells and normal cells. These probes represent two first-generation chemodosimeters featuring enzyme-mediated rapid, irreversible aromatic hydrocarbon transfer between the sulfur and nitrogen atoms accompanied by switching of photophysical properties. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24460]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wang, FY,Zhu, Y,Zhou, L,et al. Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific gamma-Glutamyltranspeptidase[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015,54(25):7349—7353. |
| APA | Wang, FY.,Zhu, Y.,Zhou, L.,Pan, L.,Cui, ZF.,...&Fan, CH.(2015).Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific gamma-Glutamyltranspeptidase.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,54(25),7349—7353. |
| MLA | Wang, FY,et al."Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific gamma-Glutamyltranspeptidase".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 54.25(2015):7349—7353. |
入库方式： OAI收割
来源：上海应用物理研究所
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