Simultaneous isolation and detection of circulating tumor cells with a microfluidic silicon-nanowire-array integrated with magnetic upconversion nanoprobes
文献类型：期刊论文
| 作者 | Wang, C; Ye, M; Cheng, L; Li, R; Zhu, WW; Shi, Z; Fan, CH; He, JK; Liu, J; Liu, Z |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2015 |
| 卷号 | 54页码:55—62 |
| 关键词 | RESISTANT PROSTATE-CANCER METASTATIC BREAST-CANCER EFFICIENT CAPTURE NANOPARTICLES PROGRESSION NANOMATERIALS SURVIVAL PLATFORM THERAPY RELEASE |
| 英文摘要 | The development of sensitive and convenient methods for detection, enrichment, and analysis of circulating tumor cells (CTCs), which serve as an importance diagnostic indicator for metastatic progression of cancer, has received tremendous attention in recent years. In this work, a new approach characteristic of simultaneous CTC capture and detection is developed by integrating a microfluidic silicon nanowire (SiNW) array with multifunctional magnetic upconversion nanoparticles (MUNPs). The MUNPs were conjugated with anti-EpCAM antibody, thus capable to specifically recognize tumor cells in the blood samples and pull them down under an external magnetic field. The capture efficiency of CTCs was further improved by the integration with a microfluidic SiNW array. Due to the autofluorescence free nature in upconversion luminescence (UCL) imaging, our approach allows for highly sensitive detection of small numbers of tumor cells, which afterward could be collected for further analysis and re-culturing. We have further demonstrated that this approach can be applied to detect CTCs in clinical blood samples from lung cancer patients, and obtained consistent results by analyzing the UCL signals and the clinical outcomes of lung cancer metastasis. Therefore our approach represents a promising platform in CTC capture and detection with potential clinical utilization in cancer diagnosis and prognosis. (C) 2015 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24567]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wang, C,Ye, M,Cheng, L,et al. Simultaneous isolation and detection of circulating tumor cells with a microfluidic silicon-nanowire-array integrated with magnetic upconversion nanoprobes[J]. BIOMATERIALS,2015,54:55—62. |
| APA | Wang, C.,Ye, M.,Cheng, L.,Li, R.,Zhu, WW.,...&Liu, Z.(2015).Simultaneous isolation and detection of circulating tumor cells with a microfluidic silicon-nanowire-array integrated with magnetic upconversion nanoprobes.BIOMATERIALS,54,55—62. |
| MLA | Wang, C,et al."Simultaneous isolation and detection of circulating tumor cells with a microfluidic silicon-nanowire-array integrated with magnetic upconversion nanoprobes".BIOMATERIALS 54(2015):55—62. |
入库方式： OAI收割
来源：上海应用物理研究所
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