Unraveling the Role of Hydrogen Peroxide in alpha-Synuclein Aggregation Using an Ultrasensitive Nanoplasmonic Probe
文献类型：期刊论文
| 作者 | Xu, Y; Li, K; Qin, WW; Zhu, B; Zhou, Z; Shi, JY; Wang, K; Hu, J; Fan, CH; Li, D |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2015 |
| 卷号 | 87期号:3页码:1968—1973 |
| 关键词 | PARKINSONS-DISEASE GOLD NANOPARTICLES AU NANOPARTICLES CELL-DEATH IN-VITRO H2O2 POLYANILINE BIOSENSORS HYBRIDIZATION PATHWAYS |
| 英文摘要 | Aggregation of alpha-Synuclein (alpha-Syn) in Lewy bodies is largely responsible for the demise and death of dopamine neurons. Oxidative stress associated with the aggregation-induced oxidative damage is considered as a possible origin of the toxicity. However, the cellular mechanism of H2O2 in the aggregation of alpha-Syn remains a debate, i.e., whether the aggregation is caused by endogenously secreted or exogenous H2O2 from upstream. Here, we report on the development of an ultrasensitive plasmonic assay with a designed nanoplasmonic probe to unravel the role of H2O2 in the aggregation of alpha-Syn. The nanoplasmonic probe is composed of a Au nanoparticle with surface-attached double-stranded DNA and horseradish peroxidase (HRP). In the presence of H2O2, HRP initiates the polymerization of aniline, which in turn results in the in situ formation of a layer of conducting polymer on the nanoplasmonic probe. By monitoring the associated plasmonic response, we can sensitively detect H2O2 with a remarkably low detection limit of 8 nM. With this ultrasensitive plasmonic assay, we find that exogenous H2O2 plays a dominant role for the aggregation of alpha-Syn in vitro, whereas the contribution from endogenously secreted H2O2 is negligible. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/24620]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Xu, Y,Li, K,Qin, WW,et al. Unraveling the Role of Hydrogen Peroxide in alpha-Synuclein Aggregation Using an Ultrasensitive Nanoplasmonic Probe[J]. ANALYTICAL CHEMISTRY,2015,87(3):1968—1973. |
| APA | Xu, Y.,Li, K.,Qin, WW.,Zhu, B.,Zhou, Z.,...&Li, D.(2015).Unraveling the Role of Hydrogen Peroxide in alpha-Synuclein Aggregation Using an Ultrasensitive Nanoplasmonic Probe.ANALYTICAL CHEMISTRY,87(3),1968—1973. |
| MLA | Xu, Y,et al."Unraveling the Role of Hydrogen Peroxide in alpha-Synuclein Aggregation Using an Ultrasensitive Nanoplasmonic Probe".ANALYTICAL CHEMISTRY 87.3(2015):1968—1973. |
入库方式： OAI收割
来源：上海应用物理研究所
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