Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer
文献类型：期刊论文
| 作者 | Zheng Feng ; Qin Yejun ; Chen Kun |
| 刊名 | j. biomed. opt.
 |
| 出版日期 | 2007 |
| 卷号 | 12期号:3页码:34002 |
| 关键词 | laser tweezers Raman spectroscopy colorectal cancer artificial neural network sensitivity map |
| ISSN号 | 1083-3668 |
| 中文摘要 | raman spectroscopy on single, living epithelial cells captured in a laser trap is shown to have diagnostic power over colorectal cancer. this new single-cell technology comprises three major components: primary culture processing of human tissue samples to produce single-cell suspensions, raman detection on singly trapped cells, and diagnoses of the cells by artificial neural network classifications. it is compared with dna flow cytometry for similarities and differences. its advantages over tissue raman spectroscopy are also discussed. in the actual construction of a diagnostic model for colorectal cancer, real patient data were taken to generate a training set of 320 raman spectra and, a test set of 80. by incorporating outlier corrections to a conventional binary neural classifier, our network accomplished significantly better predictions than logistic regressions, with sensitivity improved from 77.5% to 86.3% and specificity improved from 81.3% to 86.3% for the training set and moderate improvements for the test set. most important, the network approach enables a sensitivity map analysis to quantitate the relevance of each raman band to the normal-to-cancer transform at the cell level. our technique has direct clinic applications for diagnosing cancers and basic science potential in the study of cell dynamics of carcinogenesis. (c) 2007 society of photo-optical instrumentation engineers. |
| 学科主题 | 光学;量子光学 |
| 语种 | 英语 |
| 公开日期 | 2009-09-18 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/1101]  |
| 专题 | 上海光学精密机械研究所_量子光学重点实验室 |
| 推荐引用方式 GB/T 7714 | Zheng Feng,Qin Yejun,Chen Kun. Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer[J]. j. biomed. opt.,2007,12(3):34002. |
| APA | Zheng Feng,Qin Yejun,&Chen Kun.(2007).Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer.j. biomed. opt.,12(3),34002. |
| MLA | Zheng Feng,et al."Sensitivity map of laser tweezers Raman spectroscopy for single-cell analysis of colorectal cancer".j. biomed. opt. 12.3(2007):34002. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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