Study of the effect of alcohol on single human red blood cells using near-infrared laser tweezers Raman spectroscopy
文献类型:期刊论文
作者 | Deng JL(邓见辽) ; Wei Q ; Zhang MH ; Wang YZ ; Li YQ |
刊名 | j. raman spectrosc.
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出版日期 | 2005 |
卷号 | 36期号:3页码:257 |
关键词 | laser tweezers Raman spectroscopy red blood cell alcohol |
ISSN号 | 0377-0486 |
中文摘要 | the effect of alcohol solution on single human red blood cells (rbcs) was investigated using near-infrared laser tweezers raman spectroscopy (ltrs). in our system, a low-power diode laser at 785 nm was applied for the trapping of a living cell and the excitation of its raman spectrum. such a design could simultaneously reduce the photo-damage to the cell and suppress the interference from the fluorescence on the raman signal. the denaturation process of single rbcs in 20% alcohol solution was investigated by detecting the time evolution of the raman spectra at the single-cell level. the vitality of rbcs was characterized by the raman band at 752 cm(-1), which corresponds to the porphyrin breathing mode. we found that the intensity of this band decreased by 34.1% over a period of 25 min after the administration of alcohol. in a further study of the dependence of denaturation on alcohol concentration, we discovered that the decrease in the intensity of the 752 cm(-1) band became more rapid and more prominent as the alcohol concentration increased. the present ltrs technique may have several potential applications in cell biology and medicine, including probing dynamic cellular processes at the single cell level and diagnosing cell disorders in real time. copyright (c) 2005 john wiley t sons, ltd. |
学科主题 | 光学;量子光学 |
收录类别 | 0 |
语种 | 英语 |
WOS记录号 | WOS:000227746900009 |
公开日期 | 2009-09-18 |
源URL | [http://ir.siom.ac.cn/handle/181231/1121] ![]() |
专题 | 上海光学精密机械研究所_量子光学重点实验室 |
推荐引用方式 GB/T 7714 | Deng JL,Wei Q,Zhang MH,et al. Study of the effect of alcohol on single human red blood cells using near-infrared laser tweezers Raman spectroscopy[J]. j. raman spectrosc.,2005,36(3):257, 261. |
APA | 邓见辽,Wei Q,Zhang MH,Wang YZ,&Li YQ.(2005).Study of the effect of alcohol on single human red blood cells using near-infrared laser tweezers Raman spectroscopy.j. raman spectrosc.,36(3),257. |
MLA | 邓见辽,et al."Study of the effect of alcohol on single human red blood cells using near-infrared laser tweezers Raman spectroscopy".j. raman spectrosc. 36.3(2005):257. |
入库方式: OAI收割
来源:上海光学精密机械研究所
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