中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Study of the effect of alcohol on single human red blood cells using near-infrared laser tweezers Raman spectroscopy

文献类型:期刊论文

作者Deng JL(邓见辽) ; Wei Q ; Zhang MH ; Wang YZ ; Li YQ
刊名j. raman spectrosc.
出版日期2005
卷号36期号:3页码:257
关键词laser tweezers Raman spectroscopy red blood cell alcohol
ISSN号0377-0486
中文摘要the effect of alcohol solution on single human red blood cells (rbcs) was investigated using near-infrared laser tweezers raman spectroscopy (ltrs). in our system, a low-power diode laser at 785 nm was applied for the trapping of a living cell and the excitation of its raman spectrum. such a design could simultaneously reduce the photo-damage to the cell and suppress the interference from the fluorescence on the raman signal. the denaturation process of single rbcs in 20% alcohol solution was investigated by detecting the time evolution of the raman spectra at the single-cell level. the vitality of rbcs was characterized by the raman band at 752 cm(-1), which corresponds to the porphyrin breathing mode. we found that the intensity of this band decreased by 34.1% over a period of 25 min after the administration of alcohol. in a further study of the dependence of denaturation on alcohol concentration, we discovered that the decrease in the intensity of the 752 cm(-1) band became more rapid and more prominent as the alcohol concentration increased. the present ltrs technique may have several potential applications in cell biology and medicine, including probing dynamic cellular processes at the single cell level and diagnosing cell disorders in real time. copyright (c) 2005 john wiley t sons, ltd.
学科主题光学;量子光学
收录类别0
语种英语
WOS记录号WOS:000227746900009
公开日期2009-09-18
源URL[http://ir.siom.ac.cn/handle/181231/1121]  
专题上海光学精密机械研究所_量子光学重点实验室
推荐引用方式
GB/T 7714
Deng JL,Wei Q,Zhang MH,et al. Study of the effect of alcohol on single human red blood cells using near-infrared laser tweezers Raman spectroscopy[J]. j. raman spectrosc.,2005,36(3):257, 261.
APA 邓见辽,Wei Q,Zhang MH,Wang YZ,&Li YQ.(2005).Study of the effect of alcohol on single human red blood cells using near-infrared laser tweezers Raman spectroscopy.j. raman spectrosc.,36(3),257.
MLA 邓见辽,et al."Study of the effect of alcohol on single human red blood cells using near-infrared laser tweezers Raman spectroscopy".j. raman spectrosc. 36.3(2005):257.

入库方式: OAI收割

来源:上海光学精密机械研究所

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