Reversible immobilization of glucoamylase onto magnetic polystyrene beads with multifunctional groups (SCI)
文献类型：期刊论文
| 作者 | Wang J. Z. ; Wu D. M. ; Zhao G. H. ; Li M. R. ; Li Y. F. ; Han Y. ; He A. X. ; Jiang Y. |
| 刊名 | Process Biochemistry
 |
| 出版日期 | 2014 |
| 卷号 | 49期号:5页码:845-849 |
| 英文摘要 | A novel and simple process for the surface functionalization of micron-sized monodisperse magnetic polystyrene (PS) microbeads was reported. The polystyrene seed particles were prepared prior to the dispersion polymerization method. Afterwards, series of surface chemical modifications on polystyrene microspheres were conducted, and three end-functional microspheres with carboxyl, imidazolyl and sulphydryl groups were obtained. The functional magnetic polystyrene microspheres were prepared by impregnation and subsequent precipitation of ferric and ferrous ions into the polystyrene particles. Finally, the functional magnetic polystyrene was used for the reversible immobilization of glucoamylase via metal-affinity adsorption. The results indicated that the obtained immobilized glucoamylase presented excellent reusability, applicability, magnetic response and regeneration of supports. The magnetic PS microspheres retained >65% of its initial activity at 65 degrees C over 6 h; and the lowest residual activity of immobilized glucoamylase prepared by regenerated supports still remained about 50% of the initial activity after the 10th cycles. (C) 2014 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.casnw.net/handle/362004/25073]  |
| 专题 | 寒区旱区环境与工程研究所_中科院寒区旱区环境与工程研究所（未分类）_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang J. Z.,Wu D. M.,Zhao G. H.,et al. Reversible immobilization of glucoamylase onto magnetic polystyrene beads with multifunctional groups (SCI)[J]. Process Biochemistry,2014,49(5):845-849. |
| APA | Wang J. Z..,Wu D. M..,Zhao G. H..,Li M. R..,Li Y. F..,...&Jiang Y..(2014).Reversible immobilization of glucoamylase onto magnetic polystyrene beads with multifunctional groups (SCI).Process Biochemistry,49(5),845-849. |
| MLA | Wang J. Z.,et al."Reversible immobilization of glucoamylase onto magnetic polystyrene beads with multifunctional groups (SCI)".Process Biochemistry 49.5(2014):845-849. |
入库方式： OAI收割
来源：寒区旱区环境与工程研究所
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