Reference Gene Selection for Quantitative Real-Time PCR Normalization in Reaumuria soongorica (SCI)
文献类型：期刊论文
| 作者 | Yan X. ; Dong X. C. ; Zhang W. ; Yin H. X. ; Xiao H. L. ; Chen P. ; Ma X. F. |
| 刊名 | Plos One
 |
| 出版日期 | 2014 |
| 卷号 | 9期号:8页码:10 |
| 英文摘要 | Despite its superiority for evaluating gene expression, real-time quantitative polymerase chain reaction (qPCR) results can be significantly biased by the use of inappropriate reference genes under different experimental conditions. Reaumuria soongorica is a dominant species of desert ecosystems in arid central Asia. Given the increasing interest in ecological engineering and potential genetic resources for arid agronomy, it is important to analyze gene function. However, systematic evaluation of stable reference genes should be performed prior to such analyses. In this study, the stabilities of 10 candidate reference genes were analyzed under 4 kinds of abiotic stresses (drought, salt, dark, and heat) within 4 accessions (HG010, HG020, XGG030, and XGG040) from 2 different habitats using 3 algorithms (geNorm, NormFinder, and BestKeeper). After validation of the ribulose-1,5-bisphosphate carboxylase/oxygenase large unite (rbcL) expression pattern, our data suggested that histone H2A (H2A) and eukaryotic initiation factor 4A-2 (EIF4A2) were the most stable reference genes, cyclophilin (CYCL) was moderate, and elongation factor 1 alpha (EF1 alpha) was the worst choice. This first systematic analysis for stably expressed genes will facilitate future functional analyses and deep mining of genetic resources in R. soongorica and other species of the Reaumuria genus. |
| 收录类别 | SCI |
| 公开日期 | 2015-12-24 |
| 源URL | [http://ir.casnw.net/handle/362004/25133]  |
| 专题 | 寒区旱区环境与工程研究所_中科院寒区旱区环境与工程研究所（未分类）_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yan X.,Dong X. C.,Zhang W.,et al. Reference Gene Selection for Quantitative Real-Time PCR Normalization in Reaumuria soongorica (SCI)[J]. Plos One,2014,9(8):10. |
| APA | Yan X..,Dong X. C..,Zhang W..,Yin H. X..,Xiao H. L..,...&Ma X. F..(2014).Reference Gene Selection for Quantitative Real-Time PCR Normalization in Reaumuria soongorica (SCI).Plos One,9(8),10. |
| MLA | Yan X.,et al."Reference Gene Selection for Quantitative Real-Time PCR Normalization in Reaumuria soongorica (SCI)".Plos One 9.8(2014):10. |
入库方式： OAI收割
来源：寒区旱区环境与工程研究所
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