SRSF10 Regulates Alternative Splicing and Is Required for Adipocyte Differentiation
文献类型：期刊论文
| 作者 | Li, Huang; Cheng, Yuanming; Wu, Wenwu; Liu, Yuguo; Wei, Ning; Feng, Xiaoyan; Xie, Zhiqin（谢直勤）; Feng, Ying（冯英）
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| 刊名 | MOLECULAR AND CELLULAR BIOLOGY
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| 出版日期 | 2014 |
| 卷号 | 34期号:12页码:2198-2207 |
| 通讯作者 | Feng, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Food Safety Res, Shanghai, Peoples R China. |
| 英文摘要 | During adipocyte differentiation, significant alternative splicing changes occur in association with the adipogenic process. However, little is known about roles played by splicing factors in this process. We observed that mice deficient for the splicing factor SRSF10 exhibit severely impaired development of subcutaneous white adipose tissue (WAT) as a result of defects in adipogenic differentiation. To identify splicing events responsible for this, transcriptome sequencing (RNA-seq) analysis was performed using embryonic fibroblast cells. Several SRSF10-affected splicing events that are implicated in adipogenesis have been identified. Notably, lipin 1, known as an important regulator during adipogenesis, was further investigated. While lipin 1 beta is mainly involved in lipogenesis, its alternatively spliced isoform lipin 1 alpha, generated through the skipping of exon 7, is primarily required for initial adipocyte differentiation. Skipping of exon 7 is controlled by an SRSF10-regulated cis element located in the constitutive exon 8. The activity of this element depends on the binding of SRSF10 and correlates with the relative abundance of lipin 1 alpha mRNA. A series of experiments demonstrated that SRSF10 controls the production of lipin 1 alpha and thus promotes adipocyte differentiation. Indeed, lipin 1 alpha expression could rescue SRSF10-mediated adipogenic defects. Taken together, our results identify SRSF10 as an essential regulator for adipocyte differentiation and also provide new insights into splicing control by SRSF10 in lipin 1 pre-mRNA splicing. |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | ADIPOSE-TISSUE ; ENDOCRINE ORGAN ; PYRUVATE-KINASE ; IN-VIVO ; ADIPOGENESIS ; CELL ; METABOLISM ; ROLES ; ISOFORM ; CANCER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336743200008 |
| 公开日期 | 2015-12-24 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/378]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_代谢性疾病的基因表达调控研究组 |
| 推荐引用方式 GB/T 7714 | Li, Huang,Cheng, Yuanming,Wu, Wenwu,et al. SRSF10 Regulates Alternative Splicing and Is Required for Adipocyte Differentiation[J]. MOLECULAR AND CELLULAR BIOLOGY,2014,34(12):2198-2207. |
| APA | Li, Huang.,Cheng, Yuanming.,Wu, Wenwu.,Liu, Yuguo.,Wei, Ning.,...&Feng, Ying.(2014).SRSF10 Regulates Alternative Splicing and Is Required for Adipocyte Differentiation.MOLECULAR AND CELLULAR BIOLOGY,34(12),2198-2207. |
| MLA | Li, Huang,et al."SRSF10 Regulates Alternative Splicing and Is Required for Adipocyte Differentiation".MOLECULAR AND CELLULAR BIOLOGY 34.12(2014):2198-2207. |
入库方式： OAI收割
来源：上海营养与健康研究所
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