热门
Transcriptome analysis of alternative splicing events regulated by SRSF10 reveals position-dependent splicing modulation
文献类型：期刊论文
| 作者 | Zhou, Xuexia; Wu, Wenwu; Li, Huang; Cheng, Yuanming; Wei, Ning; Zong, Jie; Feng, Xiaoyan; Xie, Zhiqin（谢直勤）; Chen, Dai; Manley, James L. |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 42期号:6页码:4019-4030 |
| 通讯作者 | Feng, Y (reprint author), Chinese Acad Sci, Inst Nutr Sci, Key Lab Food Safety Res, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China. |
| 英文摘要 | Splicing factor SRSF10 is known to function as a sequence-specific splicing activator. Here, we used RNA-seq coupled with bioinformatics analysis to identify the extensive splicing network regulated by SRSF10 in chicken cells. We found that SRSF10 promoted both exon inclusion and exclusion. Motif analysis revealed that SRSF10 binding to cassette exons was associated with exon inclusion, whereas the binding of SRSF10 within downstream constitutive exons was associated with exon exclusion. This positional effect was further demonstrated by the mutagenesis of potential SRSF10 binding motifs in two minigene constructs. Functionally, many of SRSF10-verified alternative exons are linked to pathways of stress and apoptosis. Consistent with this observation, cells depleted of SRSF10 expression were far more susceptible to endoplasmic reticulum stress-induced apoptosis than control cells. Importantly, reconstituted SRSF10 in knockout cells recovered wild-type splicing patterns and considerably rescued the stress-related defects. Together, our results provide mechanistic insight into SRSF10-regulated alternative splicing events in vivo and demonstrate that SRSF10 plays a crucial role in cell survival under stress conditions. |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | HEAT-SHOCK ; SRP38 ; PROTEIN-1 ; REPRESSOR ; APOPTOSIS ; ACTIVATOR ; INSIGHTS ; DISEASE ; STRESS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334758600052 |
| 公开日期 | 2015-12-24 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/379]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_代谢性疾病的基因表达调控研究组 |
| 推荐引用方式 GB/T 7714 | Zhou, Xuexia,Wu, Wenwu,Li, Huang,et al. Transcriptome analysis of alternative splicing events regulated by SRSF10 reveals position-dependent splicing modulation[J]. NUCLEIC ACIDS RESEARCH,2014,42(6):4019-4030. |
| APA | Zhou, Xuexia.,Wu, Wenwu.,Li, Huang.,Cheng, Yuanming.,Wei, Ning.,...&Feng, Ying.(2014).Transcriptome analysis of alternative splicing events regulated by SRSF10 reveals position-dependent splicing modulation.NUCLEIC ACIDS RESEARCH,42(6),4019-4030. |
| MLA | Zhou, Xuexia,et al."Transcriptome analysis of alternative splicing events regulated by SRSF10 reveals position-dependent splicing modulation".NUCLEIC ACIDS RESEARCH 42.6(2014):4019-4030. |
入库方式： OAI收割
来源：上海营养与健康研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。