Far upstream element-binding protein 1 and RNA secondary structure both mediate second-step splicing repression
文献类型：期刊论文
| 作者 | Li, Huang; Wang, Zhijia; Zhou, Xuexia; Cheng, Yuanming; Xie, Zhiqin（谢直勤）; Manley, James L.; Feng, Ying（冯英）
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| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2013 |
| 卷号 | 110期号:29页码:E2687-E2695 |
| 通讯作者 | Manley, JL (reprint author), Columbia Univ, Dept Biol Sci, New York, NY 10027 USA. |
| 英文摘要 | Splicing of mRNA precursors consists of two steps that are almost invariably tightly coupled to facilitate efficient generation of spliced mRNA. However, we described previously a splicing substrate that is completely blocked after the first step. We have now investigated the basis for this unusual second-step inhibition and unexpectedly elucidated two independent mechanisms. One involves a stem-loop structure located downstream of the 3' splice site, and the other involves an exonic splicing silencer (ESS) situated 3' to the structure. Both elements contribute to the second-step block in vitro and also cause exon skipping in vivo. Importantly, we identified far upstream element-binding protein 1 (FUBP1), a single-stranded DNA- and RNA-binding protein not previously implicated in splicing, as a strong ESS binding protein, and several assays implicate it in ESS function. We demonstrate using depletion/add-back experiments that FUBP1 acts as a second-step repressor in vitro and show by siRNA-mediated knockdown and overexpression assays that it modulates exon inclusion in vivo. Together, our results provide additional insights into splicing control, and identify FUBP1 as a splicing regulator. |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | PRE-MESSENGER-RNA ; CELL LUNG-CANCER ; C-MYC ; REGULATORY PROTEIN ; EXON DEFINITION ; HU PROTEINS ; SEQUENCE ; IDENTIFICATION ; RECOGNITION ; SPLICEOSOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000322086100007 |
| 公开日期 | 2015-12-24 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/380]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_代谢性疾病的基因表达调控研究组 |
| 推荐引用方式 GB/T 7714 | Li, Huang,Wang, Zhijia,Zhou, Xuexia,et al. Far upstream element-binding protein 1 and RNA secondary structure both mediate second-step splicing repression[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(29):E2687-E2695. |
| APA | Li, Huang.,Wang, Zhijia.,Zhou, Xuexia.,Cheng, Yuanming.,Xie, Zhiqin.,...&Feng, Ying.(2013).Far upstream element-binding protein 1 and RNA secondary structure both mediate second-step splicing repression.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(29),E2687-E2695. |
| MLA | Li, Huang,et al."Far upstream element-binding protein 1 and RNA secondary structure both mediate second-step splicing repression".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.29(2013):E2687-E2695. |
入库方式： OAI收割
来源：上海营养与健康研究所
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