Deficiency in RNA editing enzyme ADAR2 impairs regulated exocytosis
文献类型：期刊论文
| 作者 | Yang, Liu（杨柳）; Zhao, Liyun; Gan, Zhenji; He, Zixuan; Xu, Jingyue; Gao, Xiang; Wang, Xiaorui; Han, Weiping; Chen, Liangyi; Xu, Tao |
| 刊名 | FASEB JOURNAL
 |
| 出版日期 | 2010 |
| 卷号 | 24期号:10页码:3720-3732 |
| 关键词 | GSIS adenosine deaminase pancreatic beta cells PC12 cells |
| 通讯作者 | Li, WJ (reprint author), Chinese Acad Sci, Inst Nutr Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab, Shanghai 200031, Peoples R China. |
| 英文摘要 | Mammalian RNA editing catalyzed by adenosine deaminases acting on RNA (ADARs) ADAR1 and ADAR2 plays pivotal roles in the brain through functional modifications of neurotransmitter receptors and ion channels. We have demonstrated previously that RNA editing by ADAR2 is regulated metabolically in pancreatic beta cells. To investigate the cellular functions of ADAR2 in professional secretory cells, we studied the effects of ADAR2 knockdown on regulated exocytosis. Selective knockdown of ADAR2 expression markedly impaired glucose-stimulated insulin secretion in the rat insulinoma INS-1 cells and primary pancreatic islets and significantly diminished KC1-stimulated secretion of exogenous human growth hormone or endogenous chromogranin B protein in the rat adrenal pheochromocytoma PC12 cells. Notably, restored overexpression of catalytically active but not editing-deficient mutant ADAR2 could rescue the impairment in stimulated secretion from ADAR2 knockdown cells. Moreover, ADAR2 suppression significantly attenuated Ca2(+) -evoked membrane capacitance increases and appreciably reduced the number of membrane-docked insulin granules in INS-1 cells. Interestingly, the secretory defects resulting from ADAR2 deficiency were coupled to decreased expression of Munc18-1 and synaptotagmin-7, two key molecules in the regulation of vesicle exocytosis. Thus, these findings reveal an important aspect of ADAR2 actions in regulated exocytosis, implicating RNA editing in the control of cellular secretory machinery.-Yang, L., Zhao, L., Gan, Z., He, Z., Xu, J., Gao, X., Wang, X., Han, W., Chen, L., Xu, T., Li, W., Liu, Y. Deficiency in RNA editing enzyme ADAR2 impairs regulated exocytosis. FASEB J. 24, 3720-3732 (2010). www.fasebj.org |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biology ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| 关键词[WOS] | PANCREATIC BETA-CELLS ; SENSITIVE K+ CHANNEL ; NEURONAL SNARE COMPLEX ; INSULIN-SECRETION ; ADENOSINE-DEAMINASE ; GRANULE EXOCYTOSIS ; RECEPTOR CHANNELS ; RAT-BRAIN ; GLUTAMATE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285005900012 |
| 公开日期 | 2015-12-24 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/291]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_糖脂代谢与调控研究组 |
| 推荐引用方式 GB/T 7714 | Yang, Liu,Zhao, Liyun,Gan, Zhenji,et al. Deficiency in RNA editing enzyme ADAR2 impairs regulated exocytosis[J]. FASEB JOURNAL,2010,24(10):3720-3732. |
| APA | Yang, Liu.,Zhao, Liyun.,Gan, Zhenji.,He, Zixuan.,Xu, Jingyue.,...&Liu, Yong.(2010).Deficiency in RNA editing enzyme ADAR2 impairs regulated exocytosis.FASEB JOURNAL,24(10),3720-3732. |
| MLA | Yang, Liu,et al."Deficiency in RNA editing enzyme ADAR2 impairs regulated exocytosis".FASEB JOURNAL 24.10(2010):3720-3732. |
入库方式： OAI收割
来源：上海营养与健康研究所
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