Deficiency in hepatic ATP-citrate lyase affects VLDL-triglyceride mobilization and liver fatty acid composition in mice
文献类型：期刊论文
| 作者 | Wang, Qiong; Li, Shoufeng; Jiang, Lei; Zhou, Yunhua; Li, Zi; Shao, Mengle; Li, Wenjun; Liu, Yong（刘勇）
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| 刊名 | JOURNAL OF LIPID RESEARCH
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| 出版日期 | 2010 |
| 卷号 | 51期号:9页码:2516-2526 |
| 关键词 | hypotriglyceridemia apolipoprotein B diet-induced obesity |
| 通讯作者 | Liu, Y (reprint author), Chinese Acad Sci, Key Lab Nutr & Metab, Inst Nutr Sci, Shanghai Inst Biol Sci, Shanghai, Peoples R China. |
| 英文摘要 | ATP-citrate lyase (ACL) is a key lipogenic enzyme that converts citrate in the cytoplasm to acetyl-CoA, the initial precursor that yields malonyl-CoA for fatty acid biosynthesis. As cytosolic citrate is derived from the tricarboxylic acid cycle in the mitochondrion, ACL catalyzes a critical reaction linking cellular glucose catabolism and lipid synthesis. To investigate the metabolic action of ACL in lipid homeostasis, we specifically knocked down hepatic ACL expression by adenovirus-mediated RNA interference in mice maintained on a low-fat or high-fat diet. Hepatic ACL abrogation markedly reduced the liver abundance of both acetylCoA and malonyl-CoA regardless of dietary fat intake, which was paralleled with decreases in circulating levels of triglycerides and free fatty acids. Moreover, hepatic ACL knockdown resulted in diet-dependent changes in the expression of other lipogenic enzymes, accompanied by altered fatty acid compositions in the liver. Interestingly, ACL deficiency led to reduced serum VLDL-triglyceride levels but increased hepatic triglyceride content, resulting at least partially from decreased hepatic secretion of VLDL-containing apolipoprotein B-48. Together, these results demonstrate that hepatic ACL suppression exerts profound effects on triglyceride mobilization as well as fatty acid compositions in the liver, suggesting an important role for ACL in lipid metabolism.-Wang, Q., S. Li, L. Jiang, Y. Zhou, Z. Li, M. Shao, W. Li, and Y. Liu. Deficiency in hepatic ATP-citrate lyase affects VLDL-triglyceride mobilization and liver fatty acid composition in mice. J. Lipid Res. 2010. 51: 2516-2526. |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | STEAROYL-COA DESATURASE-1 ; ANTISENSE OLIGONUCLEOTIDE INHIBITORS ; INSULIN-RESISTANCE ; APOLIPOPROTEIN-B ; GLUCOSE-METABOLISM ; GENE-EXPRESSION ; CLEAVAGE ENZYME ; ADIPOSE-TISSUE ; PROTECTS MICE ; CELL-GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280800200004 |
| 公开日期 | 2015-12-24 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/292]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_糖脂代谢与调控研究组 |
| 推荐引用方式 GB/T 7714 | Wang, Qiong,Li, Shoufeng,Jiang, Lei,et al. Deficiency in hepatic ATP-citrate lyase affects VLDL-triglyceride mobilization and liver fatty acid composition in mice[J]. JOURNAL OF LIPID RESEARCH,2010,51(9):2516-2526. |
| APA | Wang, Qiong.,Li, Shoufeng.,Jiang, Lei.,Zhou, Yunhua.,Li, Zi.,...&Liu, Yong.(2010).Deficiency in hepatic ATP-citrate lyase affects VLDL-triglyceride mobilization and liver fatty acid composition in mice.JOURNAL OF LIPID RESEARCH,51(9),2516-2526. |
| MLA | Wang, Qiong,et al."Deficiency in hepatic ATP-citrate lyase affects VLDL-triglyceride mobilization and liver fatty acid composition in mice".JOURNAL OF LIPID RESEARCH 51.9(2010):2516-2526. |
入库方式： OAI收割
来源：上海营养与健康研究所
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