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RNA editing by ADAR2 is metabolically regulated in pancreatic islets and beta-cells

文献类型：期刊论文

作者Gan, Zhenji; Zhao, Liyun; Yang, Liu（杨柳）; Huang, Ping; Zhao, Feng; Li, Wenjun; Liu, Yong（刘勇）
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
出版日期2006
卷号281期号:44页码:33386-33394
通讯作者Liu, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Grad Sch, Shanghai 200031, Peoples R China.
英文摘要RNA editing via the conversion of adenosine (A) to inosine (I) is catalyzed by two major families of adenosine deaminases acting on RNA (ADARs), ADAR1 and ADAR2. This genetic recoding process is known to play essential roles in the brain, due in part to changes in functional activities of edited neurotransmitter receptors and ion channels. Little is known, however, about the physiological regulation and function of A to I RNA editing in peripheral tissues and other biological processes. Here, we report that both ADAR1 and ADAR2 are expressed in the murine pancreatic islets, and ADAR2 is primarily localized in the islet endocrine cells. In contrast to ADAR1, ADAR2 transcripts in the pancreatic islets exhibit a nearly 2-fold increase in insulin-resistant mice chronically fed a high fat diet. Concurrent with this diet-induced metabolic stress, RNA editing in the islets is dramatically enhanced for the RNA transcripts encoding the ionotropic glutamate receptor subunit B. Moreover, ADAR2 protein expression is repressed in the islets under fuel deficiency condition during fasting, and this repression can be completely reversed by refeeding. We also show that, specifically in pancreatic beta-cell lines, not only the expression of ADAR2 but also the glutamate receptor subunit B editing and ADAR2 self-editing are markedly augmented in response to glucose at the physiological concentration for insulin secretion stimulation. Thus, RNA editing by ADAR2 in pancreatic islets and beta-cells is metabolically regulated by nutritional and energy status, suggesting that A to I RNA editing is most likely involved in the modulation of pancreatic islet and beta-cell function.
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]ADENOSINE-DEAMINASE ADAR1 ; SPLICE-SITE VARIANTS ; PRE-MESSENGER-RNA ; GLUTAMATE-RECEPTOR ; INSULIN-SECRETION ; BINDING DOMAINS ; INTERFERON ; GLUCOSE ; EXPRESSION ; RESISTANCE
收录类别SCI
语种英语
WOS记录号WOS:000241621400048
公开日期2015-12-24
版本出版稿
源URL[http://202.127.25.144/handle/331004/300]  
专题中国科学院上海生命科学研究院营养科学研究所_糖脂代谢与调控研究组
推荐引用方式
GB/T 7714		 Gan, Zhenji,Zhao, Liyun,Yang, Liu,et al. RNA editing by ADAR2 is metabolically regulated in pancreatic islets and beta-cells[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2006,281(44):33386-33394.
APA Gan, Zhenji.,Zhao, Liyun.,Yang, Liu.,Huang, Ping.,Zhao, Feng.,...&Liu, Yong.(2006).RNA editing by ADAR2 is metabolically regulated in pancreatic islets and beta-cells.JOURNAL OF BIOLOGICAL CHEMISTRY,281(44),33386-33394.
MLA Gan, Zhenji,et al."RNA editing by ADAR2 is metabolically regulated in pancreatic islets and beta-cells".JOURNAL OF BIOLOGICAL CHEMISTRY 281.44(2006):33386-33394.

入库方式： OAI收割

来源：上海营养与健康研究所

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