Muscle-specific E3 ubiquitin ligases are involved in muscle atrophy of cancer cachexia: An in vitro and in vivo study
文献类型：期刊论文
| 作者 | Yuan, Lei; Han, Jun; Meng, Qingyang; Xi, Qiulei; Zhuang, Qiulin; Jiang, Yi; Han, Yusong; Zhang, Bo; Fang, Jing（方靖）; Wu, Guohao |
| 刊名 | ONCOLOGY REPORTS
 |
| 出版日期 | 2015 |
| 卷号 | 33期号:5页码:2261-2268 |
| 关键词 | cancer cachexia muscle atrophy atrogin-1 MuRF-1 tumor necrosis factor-alpha small interfering RNA |
| 通讯作者 | Wu, GH (reprint author), Fudan Univ, Zhongshan Hosp, Dept Gen Surg, 180 Fenglin Rd, Shanghai 200032, Peoples R China. |
| 英文摘要 | Muscle atrophy F-Box (MAFbx)/atrogin-1 and muscle ring-finger-1 (MuRF-1) have been identified as two muscle-specific E3 ubiquitin ligases that are highly expressed in skeletal muscle during muscle atrophy. However, the role of muscle-specific E3 ubiquitin ligases during the process of muscle atrophy of cancer cachexia remains largely unknown. In the present study, we analyzed the expression of atrogin-1 and MuRF-1 in the skeletal muscle of patients with malignant and benign disease. The possible mechanisms were studied both in a colon 26-induced cancer cachexia mouse model and in tumor necrosis factor-alpha (TNF-alpha) induced atrophy C2C12 cells. Our results demonstrated that atrogin-1 and MuRF-1 tended to be increased in the skeletal muscle of patients with malignant disease even before weight loss. Non-tumor body weights and gastrocnemius weights were significantly decreased while expression levels of ubiquitin proteasome pathway associated genes (atrogin-1, MuRF-1, ubiquitin and E2-14K) were upregulated in cancer cachexia mice. Significant myotube atrophy with atrogin-1 overexpression was observed in the C2C12 cells treated with TNF-alpha. Meanwhile, knockdown of atrogin-1 by small interfering RNA (siRNA) protected C2C12 cells from the adverse effect of TNF-alpha. In conclusion, muscle-specific E3 ubiquitin ligases were upregulated during cancer cachexia, and atrogin-1 may be a potential molecular target for treating muscle atrophy induced by cancer cachexia. |
| 类目[WOS] | Oncology |
| 研究领域[WOS] | Oncology |
| 关键词[WOS] | HUMAN SKELETAL-MUSCLE ; GASTRIC-CANCER ; EXPRESSION ; ANOREXIA ; ADENOCARCINOMA ; ATROGIN-1 ; PATHWAY ; GROWTH ; ALPHA ; MURF1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000353180900021 |
| 公开日期 | 2015-12-24 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/329]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_营养与癌症研究组 |
| 推荐引用方式 GB/T 7714 | Yuan, Lei,Han, Jun,Meng, Qingyang,et al. Muscle-specific E3 ubiquitin ligases are involved in muscle atrophy of cancer cachexia: An in vitro and in vivo study[J]. ONCOLOGY REPORTS,2015,33(5):2261-2268. |
| APA | Yuan, Lei.,Han, Jun.,Meng, Qingyang.,Xi, Qiulei.,Zhuang, Qiulin.,...&Wu, Guohao.(2015).Muscle-specific E3 ubiquitin ligases are involved in muscle atrophy of cancer cachexia: An in vitro and in vivo study.ONCOLOGY REPORTS,33(5),2261-2268. |
| MLA | Yuan, Lei,et al."Muscle-specific E3 ubiquitin ligases are involved in muscle atrophy of cancer cachexia: An in vitro and in vivo study".ONCOLOGY REPORTS 33.5(2015):2261-2268. |
入库方式： OAI收割
来源：上海营养与健康研究所
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