pVHL Mediates K63-Linked Ubiquitination of nCLU
文献类型：期刊论文
| 作者 | Xue, Jing; Lv, Dan-dan; Jiao, Shi; Zhao, Wenting; Li, Xuebing; Sun, Heng; Yan, Bing; Fan, li（范莉）; Hu, Rong-gui; Fang, Jing（方靖） |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012 |
| 卷号 | 7期号:4页码:e35848 |
| 通讯作者 | Fang, J (reprint author), Chinese Acad Sci, SIBS, Inst Nutr Sci, Key Lab Nutr & Metab, Shanghai, Peoples R China. |
| 英文摘要 | pVHL, product of von Hippel-Lindau (VHL) tumor suppressor gene, functions as the substrate recognition component of an E3-ubiquitin ligase that targets proteins for ubiquitination and proteasomal degradation. Hypoxia-inducible factor alpha (HIF alpha) is the well-known substrate of pVHL. Besides HIF alpha, pVHL also binds to many other proteins and has multiple functions. In this manuscript, we report that the nuclear clusterin (nCLU) is a target of pVHL. We found that pVHL had a direct interaction with nCLU. nCLU bound to pVHL at pVHL's beta domain, the site for recognition of substrate, indicating that nCLU might be a substrate of pVHL. Interestingly, pVHL bound to nCLU but did not lead to nCLU destruction. Further studies indicated that pVHL mediated K63-linked ubiquitination of nCLU and promoted nCLU nuclear translocation. In summary, our results disclose a novel function of pVHL that mediates K63-linked ubiquitination and identify nCLU as a new target of pVHL. |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | LINDAU TUMOR-SUPPRESSOR ; PROLINE HYDROXYLATION ; PROTEIN-KINASE ; CANCER CELLS ; HIF-ALPHA ; CLUSTERIN ; COMPLEX ; LIGASE ; UBIQUITYLATION ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305339200118 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/340]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_营养与癌症研究组 |
| 推荐引用方式 GB/T 7714 | Xue, Jing,Lv, Dan-dan,Jiao, Shi,et al. pVHL Mediates K63-Linked Ubiquitination of nCLU[J]. PLOS ONE,2012,7(4):e35848. |
| APA | Xue, Jing.,Lv, Dan-dan.,Jiao, Shi.,Zhao, Wenting.,Li, Xuebing.,...&Fang, Jing.(2012).pVHL Mediates K63-Linked Ubiquitination of nCLU.PLOS ONE,7(4),e35848. |
| MLA | Xue, Jing,et al."pVHL Mediates K63-Linked Ubiquitination of nCLU".PLOS ONE 7.4(2012):e35848. |
入库方式： OAI收割
来源：上海营养与健康研究所
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