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Arsenic promotes angiogenesis in vitro via a heme oxygenase-1-dependent mechanism
文献类型：期刊论文
| 作者 | Meng, Dan; Wang, Xin; Chang, Qingshan; Hitron, Andrew; Zhang, Zhuo; Xu, Mei; Chen, Gang; Luo, Jia; Jiang, Binghua; Fang, Jing（方靖） |
| 刊名 | TOXICOLOGY AND APPLIED PHARMACOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 244期号:3页码:291-299 |
| 关键词 | Arsenic Endothelial Angiogenesis Heme oxygenase 1 Bach1 Nrf2 |
| 通讯作者 | Shi, XL (reprint author), Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40536 USA. |
| 英文摘要 | Angiogenesis and vessel remodeling are fundamental to the pathogenesis of a number of diseases caused by environmental arsenic exposure, including tumorigenesis and cardiovascular diseases. Arsenic (AsIII) has been shown to stimulate angiogenesis and vascular remodeling in vivo. However, the exact molecular mechanisms accounting for arsenic-induced angiogenesis are not clear. The present study investigates the role of heme oxygenase-1 (HO-1) in sodium arsenite-mediated angiogenesis in vitro. Transwell assay, three-dimensional Matrigel assay, RT-PCR, ELISA and immunoblotting were used to determine cell migration, vascular tube formation, mRNA and protein expression. Chromatin immunoprecipitation and luciferase assay were applied to examine the DNA binding with protein and HO-1 transcriptional activity. Here, we report that low concentrations of arsenite (0.1-1 mu M) stimulated cell migration and vascular tube formation in human microvascular endothelial cells (HMVEC). Arsenite induced HO-1 mRNA and protein expression. Knock down of HO-1 expression decreased arsenite-induced VEGF expression, cell migration, and tube formation. We showed that arsenite promoted dissociation of Bach1 (a transcriptional repressor) from the HO-1 enhancers and increased Nrf2 binding to these elements. Site directed mutagenesis assay identified that Bach1 cysteine residues 557 and 574 were essential for the induction of HO-1 gene in response to arsenite. These findings demonstrate a role for HO-1 in arsenite-mediated angiogenesis in vitro. (C) 2010 Elsevier Inc. All rights reserved. |
| 类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 研究领域[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 关键词[WOS] | TRANSCRIPTIONAL REPRESSOR BACH1 ; SMOOTH-MUSCLE-CELLS ; ENDOTHELIAL-CELLS ; GENE-EXPRESSION ; CARBON-MONOXIDE ; NADPH OXIDASE ; OXYGENASE ; EXPOSURE ; PROTEIN ; NEOVASCULARIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276705400006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/347]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_营养与癌症研究组 |
| 推荐引用方式 GB/T 7714 | Meng, Dan,Wang, Xin,Chang, Qingshan,et al. Arsenic promotes angiogenesis in vitro via a heme oxygenase-1-dependent mechanism[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2010,244(3):291-299. |
| APA | Meng, Dan.,Wang, Xin.,Chang, Qingshan.,Hitron, Andrew.,Zhang, Zhuo.,...&Shi, Xianglin.(2010).Arsenic promotes angiogenesis in vitro via a heme oxygenase-1-dependent mechanism.TOXICOLOGY AND APPLIED PHARMACOLOGY,244(3),291-299. |
| MLA | Meng, Dan,et al."Arsenic promotes angiogenesis in vitro via a heme oxygenase-1-dependent mechanism".TOXICOLOGY AND APPLIED PHARMACOLOGY 244.3(2010):291-299. |
入库方式： OAI收割
来源：上海营养与健康研究所
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