中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A linkage map of common carp (Cyprinus carpio) based on AFLP and microsatellite markers

文献类型:期刊论文

作者Cheng, L.1,2; Liu, L.1,2; Yu, X.1; Wang, D.1,2; Tong, J.1
刊名ANIMAL GENETICS
出版日期2010-04-01
卷号41期号:2页码:191-198
ISSN号0268-9146
关键词AFLP common carp (Cyprinus carpio) haploids linkage map microsatellite (SSR)
通讯作者Tong, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要P>Common carp (Cyprinus carpio) is an important fish for aquaculture, but genomics of this species is still in its infancy. In this study, a linkage map of common carp based on Amplified Fragment Length Polymorphism (AFLP) and microsatellite (SSR) markers has been generated using gynogenetic haploids. Of 926 markers genotyped, 151 (149 AFLPs, two SSRs) were distorted and eliminated from the linkage analyses. A total of 699 AFLP and 20 microsatellite (SSR) markers were assigned to the map, which comprised 64 linkage groups and covered 5506.9 cM Kosambi, with an average interval distance of 7.66 cM Kosambi. The normality tests on interval map distances showed a non-normal marker distribution. Visual inspection of the map distance distribution histogram showed a cluster of interval map distances on the left side of the chart, which suggested the occurrence of AFLP marker clusters. On the other hand, the lack of an obvious cluster on the right side showed that there were a few big gaps which need more markers to bridge. The correlation analysis showed a highly significant relatedness between the length of linkage group and the number of markers, indicating that the AFLP markers in this map were randomly distributed among different linkage groups. This study is helpful for research into the common carp genome and for further studies of genetics and marker-assisted breeding in this species.
英文摘要P>Common carp (Cyprinus carpio) is an important fish for aquaculture, but genomics of this species is still in its infancy. In this study, a linkage map of common carp based on Amplified Fragment Length Polymorphism (AFLP) and microsatellite (SSR) markers has been generated using gynogenetic haploids. Of 926 markers genotyped, 151 (149 AFLPs, two SSRs) were distorted and eliminated from the linkage analyses. A total of 699 AFLP and 20 microsatellite (SSR) markers were assigned to the map, which comprised 64 linkage groups and covered 5506.9 cM Kosambi, with an average interval distance of 7.66 cM Kosambi. The normality tests on interval map distances showed a non-normal marker distribution. Visual inspection of the map distance distribution histogram showed a cluster of interval map distances on the left side of the chart, which suggested the occurrence of AFLP marker clusters. On the other hand, the lack of an obvious cluster on the right side showed that there were a few big gaps which need more markers to bridge. The correlation analysis showed a highly significant relatedness between the length of linkage group and the number of markers, indicating that the AFLP markers in this map were randomly distributed among different linkage groups. This study is helpful for research into the common carp genome and for further studies of genetics and marker-assisted breeding in this species.
学科主题Agriculture, Dairy & Animal Science; Genetics & Heredity
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Agriculture, Dairy & Animal Science ; Genetics & Heredity
研究领域[WOS]Agriculture ; Genetics & Heredity
关键词[WOS]TROUT ONCORHYNCHUS-MYKISS ; QUANTITATIVE RESISTANCE ; INTERSPECIFIC HYBRID ; TETRAPLOID POTATO ; SSR MARKERS ; EVOLUTION ; GENOME ; L. ; LOCI ; FISH
资助信息NSF [30771643]; [2004CB117405]; [2010CB126305]; [2006-G55]
收录类别SCI
语种英语
WOS记录号WOS:000275204600009
公开日期2010-12-23
源URL[http://ir.ihb.ac.cn/handle/342005/13645]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Cheng, L.,Liu, L.,Yu, X.,et al. A linkage map of common carp (Cyprinus carpio) based on AFLP and microsatellite markers[J]. ANIMAL GENETICS,2010,41(2):191-198.
APA Cheng, L.,Liu, L.,Yu, X.,Wang, D.,&Tong, J..(2010).A linkage map of common carp (Cyprinus carpio) based on AFLP and microsatellite markers.ANIMAL GENETICS,41(2),191-198.
MLA Cheng, L.,et al."A linkage map of common carp (Cyprinus carpio) based on AFLP and microsatellite markers".ANIMAL GENETICS 41.2(2010):191-198.

入库方式: OAI收割

来源:水生生物研究所

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