A colorimetric assay for screening microcystin class compounds in aquatic systems
文献类型：期刊论文
| 作者 | Wong, BSF; Lam, PKS; Xu, LH; Zhang, YY; Richardson, BJ |
| 刊名 | CHEMOSPHERE
 |
| 出版日期 | 1999-02-01 |
| 卷号 | 38期号:5页码:1113-1122 |
| 关键词 | PHOSPHATASE INHIBITION ASSAY |
| ISSN号 | 0045-6535 |
| 通讯作者 | Wong, BSF, City Univ Hong Kong, Ctr Environm Sci & Technol, Tat Chee Ave, Hong Kong, Peoples R China |
| 中文摘要 | Secondary metabolites produced by water-blooming cyanobacteria in eutrophic waters include some potent hepatotoxins, These compounds also have tumour-promoting properties, attributable to their inhibition and activation of protein phosphatases and kinases respectively. The inhibitory effect of these toxins on protein phosphatases have been employed in a commonly used radiometric assay, involving the use of a P-32-labeled substrate, for the detection and quantitation of these compounds. This paper investigates and describes a colorimetric method in which the activity of protein phosphatase 2A is determined by measuring the rate of colour production from the release of yellow p-nitrophenol using p-nitrophenyl phosphate as the substrate. Results of this study suggest that the colorimetric protein phosphatase inhibition assay is a simple, inexpensive tool for screening substances that may have tumour-promoting characteristics in aquatic systems. The detection limit of the colorimetric method is comparable to the radiometric assay. (C) 1998 Elsevier Science Ltd. All rights reserved. |
| 英文摘要 | Secondary metabolites produced by water-blooming cyanobacteria in eutrophic waters include some potent hepatotoxins, These compounds also have tumour-promoting properties, attributable to their inhibition and activation of protein phosphatases and kinases respectively. The inhibitory effect of these toxins on protein phosphatases have been employed in a commonly used radiometric assay, involving the use of a P-32-labeled substrate, for the detection and quantitation of these compounds. This paper investigates and describes a colorimetric method in which the activity of protein phosphatase 2A is determined by measuring the rate of colour production from the release of yellow p-nitrophenol using p-nitrophenyl phosphate as the substrate. Results of this study suggest that the colorimetric protein phosphatase inhibition assay is a simple, inexpensive tool for screening substances that may have tumour-promoting characteristics in aquatic systems. The detection limit of the colorimetric method is comparable to the radiometric assay. (C) 1998 Elsevier Science Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Environmental Sciences |
| 类目[WOS] | Environmental Sciences |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| 关键词[WOS] | PHOSPHATASE INHIBITION ASSAY ; OKADAIC ACID ; PROTEIN ; WATER ; CYANOBACTERIA ; PURIFICATION ; AERUGINOSA ; TOXINS ; LR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000078194100015 |
| 公开日期 | 2011-02-21 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/14404]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.City Univ Hong Kong, Ctr Environm Sci & Technol, Hong Kong, Peoples R China 2.City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wong, BSF,Lam, PKS,Xu, LH,et al. A colorimetric assay for screening microcystin class compounds in aquatic systems[J]. CHEMOSPHERE,1999,38(5):1113-1122. |
| APA | Wong, BSF,Lam, PKS,Xu, LH,Zhang, YY,&Richardson, BJ.(1999).A colorimetric assay for screening microcystin class compounds in aquatic systems.CHEMOSPHERE,38(5),1113-1122. |
| MLA | Wong, BSF,et al."A colorimetric assay for screening microcystin class compounds in aquatic systems".CHEMOSPHERE 38.5(1999):1113-1122. |
入库方式： OAI收割
来源：水生生物研究所
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