Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol
文献类型：期刊论文
| 作者 | Huang, Di1,2,3; Wang, Ru1,2; Du, Wenjie4; Wang, Guanyi4; Xia, Menglei5,6 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 196期号:NOV页码:263-272 |
| 关键词 | Crude glycerol Evolutionary engineering Intracellular metabolic profiling Fumaric acid Rhizopus oryzae |
| ISSN号 | 0960-8524 |
| 英文摘要 | Rhizopus oryzae is strictly inhibited by biodiesel-based by-product crude glycerol, which results in low fumaric acid production. In this study, evolutionary engineering was employed to activate the glycerol utilization pathway for fumaric acid production. An evolved strain G80 was selected, which could tolerate and utilize high concentrations of crude glycerol to produce 14.9 g/L fumaric acid with a yield of 0.248 g/g glycerol. Key enzymes activity analysis revealed that the evolved strain displayed a significant upregulation in glycerol dissimilation, pyruvate consumption and reductive tricarboxylic acid pathways, compared with the parent strain. Subsequently, intracellular metabolic profiling analysis showed that amino acid biosynthesis, tricarboxylic acid cycle, fatty acid and stress response metabolites accounted for metabolic difference between two strains. Moreover, a glycerol fed-batch strategy was optimized to obtain the highest fumaric acid production of 25.5 g/L, significantly increased by 20.9-fold than that of the parent strain of 1.2 g/L. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | ESCHERICHIA-COLI ; SACCHAROMYCES-CEREVISIAE ; LACTATE-DEHYDROGENASE ; KLEBSIELLA-PNEUMONIAE ; PROFILING ANALYSIS ; FERMENTATION ; 1,3-PROPANEDIOL ; GLUCOSE ; STRAIN ; XYLOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361040500035 |
| 公开日期 | 2015-12-27 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19541]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Nankai Univ, TEDA, TEDA Inst Biol Sci & Biotechnol, Tianjin 300457, Peoples R China 2.Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin 300071, Peoples R China 3.Tianjin Key Lab Microbial Funct Genom, Tianjin 300457, Peoples R China 4.Tianjin Univ, Key Lab Syst Bioengn, Minist Educ, Tianjin 300072, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Di,Wang, Ru,Du, Wenjie,et al. Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol[J]. BIORESOURCE TECHNOLOGY,2015,196(NOV):263-272. |
| APA | Huang, Di,Wang, Ru,Du, Wenjie,Wang, Guanyi,&Xia, Menglei.(2015).Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol.BIORESOURCE TECHNOLOGY,196(NOV),263-272. |
| MLA | Huang, Di,et al."Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol".BIORESOURCE TECHNOLOGY 196.NOV(2015):263-272. |
入库方式： OAI收割
来源：过程工程研究所
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