Chitosan multiple addition enhances laccase production from Trametes versicolor
文献类型：期刊论文
| 作者 | Adekunle, Abiodun Emmanuel1,3; Wang, Feng1; Hu, Jianhua1,3; Ma, Anzhou2; Guo, Chen1; Zhuang, Guoqiang2; Liu, Chun-Zhao1 |
| 刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| 出版日期 | 2015-10-01 |
| 卷号 | 38期号:10页码:1973-1981 |
| 关键词 | Chitosan Laccase Multiple addition Trametes versicolor Mycelia |
| ISSN号 | 1615-7591 |
| 英文摘要 | Chitosan multiple addition strategy was developed to improve laccase production from Trametes versicolor cultures. The optimized multiple addition strategy was carried out by two-time addition of 0.1 g L-1 chitosan to a 2-day-old culture media, with 24-h interval between the treatments. Under these conditions, laccase activity of 644.9 U l(-1) was achieved on the seventh day and laccase production was improved by 93.5 % higher than the control. Chitosan treatment increased reactive oxygen species generation and extracellular protein concentration in the treated mycelia. In contrast, the inducer inhibited the mycelia growth. The result of the quantitative reverse transcription polymerase chain reaction showed that the copy number of the laccase gene transcript increased by 16.7-fold in the treated mycelia relative to the control. This study provides insight into some of the intrinsic metabolic processes involved in the upregulation of laccase production in the presence of chitosan inducer in fungal culture. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | WHITE-ROT FUNGI ; POSTHARVEST PATHOGENS ; RHIZOPUS-STOLONIFER ; ANTIFUNGAL ACTIVITY ; GENE-EXPRESSION ; CHITIN ; INDUCTION ; BACTERIAL ; DELIVERY ; DISEASES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361823900013 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19577]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Adekunle, Abiodun Emmanuel,Wang, Feng,Hu, Jianhua,et al. Chitosan multiple addition enhances laccase production from Trametes versicolor[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2015,38(10):1973-1981. |
| APA | Adekunle, Abiodun Emmanuel.,Wang, Feng.,Hu, Jianhua.,Ma, Anzhou.,Guo, Chen.,...&Liu, Chun-Zhao.(2015).Chitosan multiple addition enhances laccase production from Trametes versicolor.BIOPROCESS AND BIOSYSTEMS ENGINEERING,38(10),1973-1981. |
| MLA | Adekunle, Abiodun Emmanuel,et al."Chitosan multiple addition enhances laccase production from Trametes versicolor".BIOPROCESS AND BIOSYSTEMS ENGINEERING 38.10(2015):1973-1981. |
入库方式： OAI收割
来源：过程工程研究所
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