Molecular shape and immunogenicity of meningococcal polysaccharide group A conjugate vaccine
文献类型：期刊论文
| 作者 | Xu, Mengfang1,3; Xing, Xueqing2; Wu, Zhonghua2; Du, Yuguang1; Hu, Tao1 |
| 刊名 | VACCINE
 |
| 出版日期 | 2015-10-26 |
| 卷号 | 33期号:43页码:5815-5821 |
| 关键词 | Neisseria meningitidis Polysaccharide Conjugate vaccine SAXS Molecular shape |
| ISSN号 | 0264-410X |
| 英文摘要 | Neisseria meningitidis is a leading cause of severe bacterial infections in infants and young children. As a major virulence factor, meningococcal capsular polysaccharide (PS) is poorly immunogenic and generally does not induce immunological memory. Conjugation of PS with a carrier protein can significantly increase the PS-specific immunogenicity and induce immunological memory. It is well known that the molecular shape/size of the conjugate vaccine is important for its immunogenicity. However, little is known about the molecular shape/size of the meningococcal conjugate vaccine. A meningococcal PS ovalbumin (OVA) conjugate vaccine was prepared using cystamine as linker. Four components (P1-P4) with different molecular size were fractionated from the conjugate. Small angle X-ray scattering (SAXS) analysis revealed that the conjugate vaccine exhibited a rod-like shape similar to virus-like particles. PS-specific immunogenicity of the conjugate vaccine was related to its molecular shape and increased as a function of its molecular size. Thus, the present study provides a three-dimensional shape of the conjugate vaccine and helps to identify optimal design of a potent meningococcal conjugate vaccine. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Medicine, Research & Experimental |
| 研究领域[WOS] | Immunology ; Research & Experimental Medicine |
| 关键词[WOS] | SOLUTION SCATTERING ; IMMUNE-RESPONSES ; RESOLUTION ; PROTEINS ; CARRIER ; DESIGN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363824600013 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19755]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Mengfang,Xing, Xueqing,Wu, Zhonghua,et al. Molecular shape and immunogenicity of meningococcal polysaccharide group A conjugate vaccine[J]. VACCINE,2015,33(43):5815-5821. |
| APA | Xu, Mengfang,Xing, Xueqing,Wu, Zhonghua,Du, Yuguang,&Hu, Tao.(2015).Molecular shape and immunogenicity of meningococcal polysaccharide group A conjugate vaccine.VACCINE,33(43),5815-5821. |
| MLA | Xu, Mengfang,et al."Molecular shape and immunogenicity of meningococcal polysaccharide group A conjugate vaccine".VACCINE 33.43(2015):5815-5821. |
入库方式： OAI收割
来源：过程工程研究所
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