Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases
文献类型：期刊论文
| 作者 | Zhang, Chenxu1,2; Zhang, Rongyue3; Li, Qiang1; Huang, Yongdong1; Zhao, Lan1; Su, Zhiguo1; Gong, Fangling1; Lv, Zheng4; Song, Hongyan4; Li, Wei4 |
| 刊名 | SEPARATION AND PURIFICATION TECHNOLOGY
 |
| 出版日期 | 2015-11-05 |
| 卷号 | 154期号:NOV页码:351-358 |
| 关键词 | Octreotide Reversed-phase Separation Poly(styrene-co-divinylbenzene) |
| ISSN号 | 1383-5866 |
| 英文摘要 | Octreotide, a disulfide-cyclized eight amino acids long analogue of somatostatin, has been used to treat the symptoms associated with metastatic carcinoid tumors (flushing and diarrhea), and vasoactive intestinal peptide secreting adenomas (watery diarrhea). As composed by D-amino acids, C-terminal alcohol, and disulfide bond, octreotide can be only prepared using chemistry synthesis method, which increases difficulty of the traditional reversed-phase separation. In this paper, we report a very fast separation of octreotide from synthetic peptide crude mixtures on a poly(styrene co-divinylbenzene) (PSt)-based reversed phases prepared by a quick membrane emulsification process. In general, fourier-transformed infrared resonance (FT-IR) spectroscopy, reversed-phase high performance liquid chromatography (HPLC) combined with electrospray ionisation mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR) spectroscopy are routinely used for analyses of resultant peptides. Under the optimized conditions, the octreotide is purified from 42.89% to 99.99%. Consequently, the poly(styrene co-divinylbenzene)-based phase can provide more flexibility to improve separation efficiency for the octreotide from the synthetic peptide crude mixtures. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | AMINO-ACID ; PURIFICATION ; POLYSTYRENE ; PROTEINS ; HPLC ; SILICA ; COLUMNS ; RESINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000364245500043 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19808]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China 3.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing 102617, Peoples R China 4.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Chenxu,Zhang, Rongyue,Li, Qiang,et al. Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2015,154(NOV):351-358. |
| APA | Zhang, Chenxu.,Zhang, Rongyue.,Li, Qiang.,Huang, Yongdong.,Zhao, Lan.,...&Ma, Guanghui.(2015).Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases.SEPARATION AND PURIFICATION TECHNOLOGY,154(NOV),351-358. |
| MLA | Zhang, Chenxu,et al."Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases".SEPARATION AND PURIFICATION TECHNOLOGY 154.NOV(2015):351-358. |
入库方式： OAI收割
来源：过程工程研究所
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