Thermostable pectate lyase from Caldicellulosiruptor kronotskyensis provides an efficient addition for plant biomass deconstruction
文献类型：期刊论文
| 作者 | Su, Hong1,2; Qiu, Weihua1; Kong, Qing3; Mi, Shuofu1; Han, Yejun1 |
| 刊名 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 121期号:NOV页码:104-112 |
| 关键词 | Pectate lyase Caldicellulosiruptor Thermostable Degumming Biomass deconstruction |
| ISSN号 | 1381-1177 |
| 英文摘要 | To understand the enzymological basis for extremely thermophilic, biomass-degrading genus Caldicellulosiruptor metabolize pectin, a thermostable pectate lyase Pel-863 encoded by a gene cluster for hexose-containing polysaccharide metabolism in genome of C. kronotskyensis was studied. The pectate lyase of Caldicellulosiruptor was highly conserved and the representative Pel-863 was biochemically characterized, and the application for pectin containing biomass degradation was also studied. Pel-863 exhibited an optimal activity at 70 degrees C and pH 9.0 with Ca2+ as cofactor. It degraded polygalacturonic acid (PGA), methylated pectin and pectic biomass through endo-cleaving action. The respective V-max and K-m for Pel-863 were 172.8 U/mg and 0.60 g/L on PGA. FTIR and SEM analysis indicated that Pel-863 could remove most of pectin in hemp fiber with less damage compared to alkaline degumming. In addition, pre-digestion with Pel-863 improved glucose and xylose yield by 14.2% and 311.6% respectively for corn stalk, 6.5% and 55% for rice stalk compared with sole action of Novozymes Cellic CTec2. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Physical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | CATALYZED PROTON ABSTRACTION ; ERWINIA-CHRYSANTHEMI 3937 ; CARBON ACIDS ; FAMILY 3 ; ESCHERICHIA-COLI ; CLONING ; RECOMBINANT ; MECHANISMS ; EXPRESSION ; PECTINASES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000365050800015 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19836]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Coll Food Sci & Engn, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Hong,Qiu, Weihua,Kong, Qing,et al. Thermostable pectate lyase from Caldicellulosiruptor kronotskyensis provides an efficient addition for plant biomass deconstruction[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2015,121(NOV):104-112. |
| APA | Su, Hong,Qiu, Weihua,Kong, Qing,Mi, Shuofu,&Han, Yejun.(2015).Thermostable pectate lyase from Caldicellulosiruptor kronotskyensis provides an efficient addition for plant biomass deconstruction.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,121(NOV),104-112. |
| MLA | Su, Hong,et al."Thermostable pectate lyase from Caldicellulosiruptor kronotskyensis provides an efficient addition for plant biomass deconstruction".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 121.NOV(2015):104-112. |
入库方式： OAI收割
来源：过程工程研究所
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