Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles
文献类型：期刊论文
| 作者 | Yu, Jiemiao1,2,3; Yang, Liangrong2; Liang, Xiangfeng2; Dong, Tingting2,3; Liu, Huizhou1,2 |
| 刊名 | TALANTA
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 144期号:NOV页码:312-317 |
| ISSN号 | 0039-9140 |
| 关键词 | Photoresponsive Thrombin Aptamer Capture Release |
| 英文摘要 | In the protein separation, adsorption and desorption of target protein have been using different buffer condition. Different buffer will change the structure and activity of target protein in some cases. This work describes the use of different wavelength light for remote regulation of adsorption and desorption of target protein in the same buffer solutions. A dynamic system that captured and released protein in response to light is reported. Matrix gold nanoparticles and light-responsive affinity ligand comprising thrombin aptamer (APT15), polyethylene glycol linker, and azobenzene-modified complementary sequence were used. UV light induced a trans-cis isomerization of the azobenzene that destabilized the duplex of aptamer and azobenzene-modified complementary sequence, resulting in thrombin binding to aptamer sequence. Visible light irradiation resulted in DNA duplex rehybridization and thrombin released. Our work demonstrates that different light wavelengths effectively regulated the adsorption and desorption of thrombin in the same buffer, and this system also can capture and release prothrombin from plasma with different wavelength light. Furthermore, this method can be widely applied to a variety of different protein separation process. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | AZOBENZENE-TETHERED DNA ; AFFINITY-CHROMATOGRAPHY ; CAPILLARY CHROMATOGRAPHY ; COMBINATORIAL CHEMISTRY ; DUPLEX FORMATION ; PHOTOREGULATION ; TRANSCRIPTION ; POLYMERASE ; SELECTION ; BIND |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000363346200044 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/19758]  |
| 专题 | 过程工程研究所_湿法冶金清洁生产技术国家工程实验室 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,et al. Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles[J]. TALANTA,2015,144(NOV):312-317. |
| APA | Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,Dong, Tingting,&Liu, Huizhou.(2015).Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles.TALANTA,144(NOV),312-317. |
| MLA | Yu, Jiemiao,et al."Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles".TALANTA 144.NOV(2015):312-317. |
入库方式： OAI收割
来源：过程工程研究所
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