Silver nanoparticle-induced hemoglobin decrease involves alteration of histone 3 methylation status
文献类型：期刊论文
| 作者 | Qian Y; Zhang J; Hu QL; Xu M; Chen Y; Hu GQ(胡国庆) ; Zhao MR; Liu SJ
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| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2015-11 |
| 卷号 | 70页码:12-22 |
| 通讯作者邮箱 | zhaomr@zjut.edu.cn ; sjliu@rcees.ac.cn |
| 关键词 | Nanosilver Epigenetics Transcription Hemoglobin Histone Methylation |
| ISSN号 | 0142-9612 |
| 产权排序 | [Qian, Yi; Zhang, Jie; Xu, Ming; Chen, Yue; Liu, Sijin] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China; [Qian, Yi; Zhao, Meirong] Zhejiang Univ Technol, Coll Biol & Environm Engn, Hangzhou 310032, Zhejiang, Peoples R China; [Hu, Qinglin; Hu, Guoqing] Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, State Key Lab Nonlinear Mech, Beijing 100085, Peoples R China |
| 通讯作者 | Liu, SJ (reprint author), Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China. |
| 中文摘要 | Silver nanoparticles (nanosilver, AgNPs) have been shown to induce toxicity in vitro and in vivo; however, the molecular bases underlying the detrimental effects have not been thoroughly understood. Although there are numerous studies on its genotoxicity, only a few studies have investigated the epigenetic changes, even less on the changes of histone modifications by AgNPs. In the current study, we probed the AgNP-induced alterations to histone methylation that could be responsible for globin reduction in erythroid cells. AgNP treatment caused a significant reduction of global methylation level for histone 3 (H3) in erythroid MEL cells at sublethal concentrations, devoid of oxidative stress. The ChIP-PCR analyses demonstrated that methylation of H3 at lysine (Lys) 4 (H3K4) and Lys 79 (H3K79) on the beta-globin locus was greatly reduced. The reduction in methylation could be attributed to decreased histone methyltransferase DOT-1L and MLL levels as well as the direct binding between AgNPs to H3/H4 that provide steric hindrance to prevent methylation as predicted by the all-atom molecular dynamics simulations. This direct interaction was further proved by AgNP-mediated pull-down assay and immunoprecipitation assay. These changes, together with decreased RNA polymerase II activity and chromatin binding at this locus, resulted in decreased hemoglobin production. By contrast, Ag ion-treated cells showed no alterations in histone methylation level. Taken together, these results showed a novel finding in which AgNPs could alter the methylation status of histone. Our study therefore opens a new avenue to study the biological effects of AgNPs at sublethal concentrations from the perspective of epigenetic mechanisms. (C) 2015 Elsevier Ltd. All rights reserved. |
| 分类号 | 一类 |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Engineering ; Materials Science |
| 关键词[WOS] | BETA-GLOBIN GENE ; MOLECULAR-DYNAMICS ; ENGINEERED NANOMATERIALS ; DOT1L METHYLTRANSFERASE ; CHROMATIN MODIFICATIONS ; EPIGENETIC REGULATION ; GOLD NANOPARTICLES ; H3K79 METHYLATION ; IN-VIVO ; MECHANISMS |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1016/j.biomaterials.2015.08.015 |
| 语种 | 英语 |
| WOS记录号 | WOS:000362610600002 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/58399]  |
| 专题 | 力学研究所_非线性力学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Qian Y,Zhang J,Hu QL,et al. Silver nanoparticle-induced hemoglobin decrease involves alteration of histone 3 methylation status[J]. BIOMATERIALS,2015,70:12-22. |
| APA | Qian Y.,Zhang J.,Hu QL.,Xu M.,Chen Y.,...&Liu SJ.(2015).Silver nanoparticle-induced hemoglobin decrease involves alteration of histone 3 methylation status.BIOMATERIALS,70,12-22. |
| MLA | Qian Y,et al."Silver nanoparticle-induced hemoglobin decrease involves alteration of histone 3 methylation status".BIOMATERIALS 70(2015):12-22. |
入库方式： OAI收割
来源：力学研究所
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