Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells
文献类型：期刊论文
| 作者 | Wang CZ; Li N; Zhang C; Sun SJ(孙树津) ; Gao YX; Long M(龙勉); Gao YX(高宇欣); Wang CZ(王成之); Li N(李宁)
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| 刊名 | MICROGRAVITY SCIENCE AND TECHNOLOGY
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| 出版日期 | 2015-09 |
| 卷号 | 27期号:6页码:515-527 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| 关键词 | HL-60 PMN Effects of simulated microgravity RCCS Flow chamber |
| ISSN号 | 0938-0108 |
| 产权排序 | [Wang, Chengzhi; Li, Ning; Zhang, Chen; Sun, Shujin; Gao, Yuxin; Long, Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China; [Wang, Chengzhi; Li, Ning; Zhang, Chen; Sun, Shujin; Gao, Yuxin; Long, Mian] Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China. |
| 中文摘要 | Altered gravity, especially microgravity affects cellular functions of immune cells and can result in immune dysfunction for long-term, manned spaceflight and space exploration. The underlying mechanism, however, of sensing and responding to the gravity alteration is poorly understood. Here, a rotary cell culture system (RCCS) bioreactor was used to elucidate the effects of simulated microgravity on polymorphonuclear neutrophils (PMN)-like HL-60 cells. Alteration of cell morphology, up-regulation of (nitric oxide) NO production, enhancement of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein 1 (MCP-1) secretion, and diversity of cellular adhesion molecule expression were observed for the cells cultured in RCCS, leading to the up-regulated inflammatory immune responses and host defense. It was also indicated that such alterations in biological responses of PMNs mediated the reduced rolling velocity and decreased adhesion of PMN-like HL-60 cells on endothelial cells under shear flow. This work furthers the understandings in the effects and mechanism of microgravity on PMN functions, which are potentially helpful for optimizing the countermeasures to immune suppression in the future long-term, manned spaceflight. |
| 分类号 | 二类/Q2 |
| 类目[WOS] | Engineering, Aerospace ; Thermodynamics ; Mechanics |
| 研究领域[WOS] | Engineering ; Thermodynamics ; Mechanics |
| 关键词[WOS] | SHORT-DURATION SPACEFLIGHT ; NITRIC-OXIDE ; ENDOTHELIAL-CELLS ; PARABOLIC FLIGHT ; L-SELECTIN ; POLYMORPHONUCLEAR LEUKOCYTES ; GROWTH-FACTOR ; BONE-MARROW ; EXPRESSION ; GRAVITY |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1007/s12217-015-9473-6 |
| 语种 | 英语 |
| WOS记录号 | WOS:000366103800013 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/58369]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Wang CZ,Li N,Zhang C,et al. Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells[J]. MICROGRAVITY SCIENCE AND TECHNOLOGY,2015,27(6):515-527. |
| APA | Wang CZ.,Li N.,Zhang C.,Sun SJ.,Gao YX.,...&Li N.(2015).Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells.MICROGRAVITY SCIENCE AND TECHNOLOGY,27(6),515-527. |
| MLA | Wang CZ,et al."Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells".MICROGRAVITY SCIENCE AND TECHNOLOGY 27.6(2015):515-527. |
入库方式： OAI收割
来源：力学研究所
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