Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats
文献类型：期刊论文
| 作者 | Guo, Xiaochun1,2; Chen, Liang2,3; Chen, Jun2; Xie, Ping1,2; Li, Shangchun2,3; He, Jun2; Li, Wei2,3; Fan, Huihui1,2; Yu, Dezhao2,3; Zeng, Cheng1,2 |
| 刊名 | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 22期号:23页码:19273-19284 |
| 关键词 | Microcystin-LR-GSH/Cys Detoxification GSH pathway Liver Rat |
| ISSN号 | 0944-1344 |
| 英文摘要 | Glutathione (GSH) plays crucial roles in antioxidant defense and detoxification metabolism of microcystin-LR (MC-LR). However, the detoxification process of MC-LR in mammals remains largely unknown. This paper, for the first time, quantitatively analyzes MC-LR and its GSH pathway metabolites (MC-LR-GSH and MC-LR-Cys) in the liver of Sprague-Dawley (SD) rat after MC-LR exposure. Rats received intraperitoneal (i.p.) injection of 0.25 and 0.5 lethal dose 50 (LD50) of MC-LR with or without pretreatment of buthionine-(S,R)-sulfoximine (BSO), an inhibitor of GSH synthesis. The contents of MC-LR-GSH were relatively low during the experiment; however, the ratio of MC-LR-Cys to MC-LR reached as high as 6.65 in 0.5 LD50 group. These results demonstrated that MC-LR-GSH could be converted to MC-LR-Cys efficiently, and this metabolic rule was in agreement with the data of aquatic animals previously reported. MC-LR contents were much higher in BSO + MC-LR-treated groups than in the single MC-LR-treated groups. Moreover, the ratio of MC-LR-Cys to MC-LR decreased significantly after BSO pretreatment, suggesting that the depletion of GSH induced by BSO reduced the detoxification of MCs. Moreover, MC-LR remarkably induced liver damage, and the effects were more pronounced in BSO pretreatment groups. In conclusion, this study verifies the role of GSH in the detoxification of MC-LR and furthers our understanding of the biochemical mechanism for SD rats to counteract toxic cyanobacteria. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| 关键词[WOS] | CARP HYPOPHTHALMICHTHYS-MOLITRIX ; TANDEM MASS-SPECTROMETRY ; CYCLIC PEPTIDE TOXIN ; BIGHEAD CARP ; INHIBITORY-ACTIVITY ; OXIDATIVE STRESS ; BALB/C MICE ; IN-VIVO ; LIVER ; TOXICITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000365816000090 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27425]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China 2.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Xiaochun,Chen, Liang,Chen, Jun,et al. Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2015,22(23):19273-19284. |
| APA | Guo, Xiaochun.,Chen, Liang.,Chen, Jun.,Xie, Ping.,Li, Shangchun.,...&Zeng, Cheng.(2015).Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,22(23),19273-19284. |
| MLA | Guo, Xiaochun,et al."Quantitatively evaluating detoxification of the hepatotoxic microcystin-LR through the glutathione (GSH) pathway in SD rats".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 22.23(2015):19273-19284. |
入库方式： OAI收割
来源：水生生物研究所
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