Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer
文献类型：期刊论文
| 作者 | Huang CH; Li JX ; Tang Y; Wang CX; Hou CJ; Huo DQ; Chen YY(陈艳艳); Jin G(靳刚)
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| 刊名 | MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
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| 出版日期 | 2011 |
| 卷号 | 31期号:7页码:1609-1613 |
| 通讯作者邮箱 | huodq@cqu.edu.cn ; yychen2006@sinano.ac.cn ; gajin@imech.ac.cn |
| 关键词 | Biosensor Imaging ellipsometry Riemerella anatipestifer Detection |
| ISSN号 | 0928-4931 |
| 产权排序 | [Jin, Gang] CAS, Inst Mech, Beijing 100190, Peoples R China; [Huang, Chenghong; Hou, Changjun; Huo, Danqun] Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Ctr Bioengn, Chongqing 400030, Peoples R China; [Huang, Chenghong; Wang, Chunxia; Chen, Yanyan] CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China; [Li, Jixiang; Tang, Yu] Southwest Univ, Chongqing 402460, Peoples R China |
| 通讯作者 | Jin, G (reprint author), CAS, Inst Mech, Beijing 100190, Peoples R China. |
| 中文摘要 | In this study, a practicable method for the detection of Riemerella anatipestifer (R. anatipestifer) using biosensor based on imaging ellipsometry (BIE) is described. The method is performed by immobilizing anti-R. anatipestifer egg yolk immunoglubilin (IgY) onto modified chemistry surface to form sensing layer. Antigen captured by sensing layer can then be quantitatively measured through imaging ellipsometry in grayscale format. The results demonstrate that it can detect R. anatipestifer as low as 5.2 x 10(3) CFU/mL. Furthermore, the proposed method can realize specific discrimination for multiple serotypes of 1,2, 4, and 14 of R. anatipestifer. It has the advantages of rapid, simple, high sensitivity and low cost. (C) 2011 Elsevier B.V. All rights reserved. |
| 分类号 | 一类 |
| 类目[WOS] | Materials Science, Biomaterials |
| 研究领域[WOS] | Materials Science |
| 关键词[WOS] | PASTEURELLA-ANATIPESTIFER ; ESCHERICHIA-COLI ; IMMUNOSENSOR ; DUCKS ; VISUALIZATION ; INFECTION |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295953900051 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/58582]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Huang CH,Li JX,Tang Y,et al. Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2011,31(7):1609-1613. |
| APA | Huang CH.,Li JX.,Tang Y.,Wang CX.,Hou CJ.,...&靳刚.(2011).Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,31(7),1609-1613. |
| MLA | Huang CH,et al."Biosensor Based on Imaging Ellipsometry for Serotype-Specific Detection of Riemerella Anatipestifer".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 31.7(2011):1609-1613. |
入库方式： OAI收割
来源：力学研究所
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