Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry
文献类型：期刊论文
| 作者 | Huang CH; Li JX ; Tang Y; Chen YY(陈艳艳); Jin G(靳刚)
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| 刊名 | CURRENT APPLIED PHYSICS
 |
| 出版日期 | 2011 |
| 卷号 | 11期号:3页码:353-357 |
| 通讯作者邮箱 | yychen2006@sinano.ac.cn ; gajin@imech.ac.cn |
| 关键词 | Biosensor Imaging ellipsometry Duck hepatitis virus Detection |
| ISSN号 | 1567-1739 |
| 产权排序 | [Huang, Chenghong; Chen, Yanyan; Jin, Gang] CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China; [Li, Jixiang; Tang, Yu] Univ Southwest, Chongqing 402460, Peoples R China; [Huang, Chenghong; Jin, Gang] CAS, Inst Biophys, Beijing 100101, Peoples R China; [Jin, Gang] CAS, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; [Huang, Chenghong] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 通讯作者 | Jin, G (reprint author), CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China. |
| 中文摘要 | A novel method for the detection of duck hepatitis virus serotype1 (DHV1) using a biosensor based on imaging ellipsometry (IE) is developed. Polyclonal antibodies against DHV1 were immobilized on silicon wafers through protein A and used to capture virus particles in sample. Signals on silicon surface were acquired directly in grayscale format on an imaging ellipsometry image. This study demonstrated that the assay is rapid and accurate for detection of duck hepatitis virus. Compared with traditional methods, this technique not only has high sensitivity as low as 8 x 10(-9.5) LD(50)/ml, but can also simultaneously perform multiple serotype identification of DHV1, DHV1-g and DHV1-s. These results suggest that this biosensor might be a practicable diagnostic tool for duck hepatitis virus detection. (C) 2010 Elsevier B.V. All rights reserved. |
| 分类号 | 二类 |
| 类目[WOS] | Materials Science, Multidisciplinary ; Physics, Applied |
| 研究领域[WOS] | Materials Science ; Physics |
| 关键词[WOS] | VISUALIZATION |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288183300017 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/58588]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Huang CH,Li JX,Tang Y,et al. Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry[J]. CURRENT APPLIED PHYSICS,2011,11(3):353-357. |
| APA | Huang CH,Li JX,Tang Y,陈艳艳,&靳刚.(2011).Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry.CURRENT APPLIED PHYSICS,11(3),353-357. |
| MLA | Huang CH,et al."Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry".CURRENT APPLIED PHYSICS 11.3(2011):353-357. |
入库方式： OAI收割
来源：力学研究所
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