A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry
文献类型：期刊论文
| 作者 | Huang CH; Chen YY(陈艳艳) ; Jin G(靳刚)
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| 刊名 | ANNALS OF BIOMEDICAL ENGINEERING
 |
| 出版日期 | 2011 |
| 卷号 | 39期号:1页码:185-192 |
| 通讯作者邮箱 | huang1ng@tom.com ; yychen2006@sinano.ac.cn ; gajin@imech.ac.cn |
| 关键词 | Biosensor Imaging ellipsometry Carbohydrate antigen 19-9 Immunoassay |
| ISSN号 | 0090-6964 |
| 产权排序 | [Huang, Chenghong; Chen, Yanyan; Jin, Gang] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China; [Huang, Chenghong; Jin, Gang] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; [Jin, Gang] CAS, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; [Huang, Chenghong] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 通讯作者 | Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China. |
| 中文摘要 | Cancer markers can offer much more information for developing or developed cancers. Simple and short-time assay of cancer biomarker is essential for clinic diagnosis and management. In this study, a one-step immunoassay for carbohydrate antigen 19-9 (CA19-9) by biosensor based on imaging ellipsometry (BIE) is described and its analysis performances are evaluated. Anti-CA19-9 monoclonal antibody as ligand covalently immobilized on carboxyl-modified silicon substrate and its immobilization concentration concurrently screened. Then, bovine serum albumin (BSA) is used to block for acquisition of sensing layer. The detection linear range is from 15.0 to 120.0 U/mL with a minimum limit of 10.0 U/mL (S/N = 3). The intra-slide and inter-slide reproducibility, expressed as coefficient variation (CV%), are 9.9, 5.9, 5.4% and 9.6, 7.6, 5.3% for 31.9, 57.4, and 99.2 U/mL of CA19-9, respectively. Accuracy determined by spiked-recovery is among 95.1-106.7%. Specificity expressed as cross-reaction rate are all below 5.8% evaluated by three analogous biomarkers alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), and carbohydrate antigen 242 (CA242). Application of BIE to 25 clinic samples demonstrated that the results are in high correlate with electrochemiluminescent immunoassay (ECLIA, R-2 = 0.997). The proposed immunoassay has the potential for clinic application. |
| 分类号 | 二类/Q2 |
| 类目[WOS] | Engineering, Biomedical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | OPTICAL PROTEIN-CHIP ; SPECTROSCOPIC ELLIPSOMETRY ; COLORECTAL-CANCER ; IMMOBILIZATION ; IMMUNOSENSOR ; MICROARRAYS ; SURFACE ; VISUALIZATION ; LAYERS ; ASSAY |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287213100017 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/58591]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Huang CH,Chen YY,Jin G. A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry[J]. ANNALS OF BIOMEDICAL ENGINEERING,2011,39(1):185-192. |
| APA | Huang CH,陈艳艳,&靳刚.(2011).A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry.ANNALS OF BIOMEDICAL ENGINEERING,39(1),185-192. |
| MLA | Huang CH,et al."A One-Step Immunoassay for Carbohydrate Antigen 19-9 By Biosensor Based on Imaging Ellipsometry".ANNALS OF BIOMEDICAL ENGINEERING 39.1(2011):185-192. |
入库方式： OAI收割
来源：力学研究所
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