Identification of A Residue in Helix 2 of Rice Plasma Membrane Intrinsic Proteins That Influences Water Permeability
文献类型：期刊论文
| 作者 | Zhang MH; Lv SQ(吕守芹) ; Li GW; Mao ZL; Yu X; Sun WN; Tang ZC; Long M(龙勉); Su WA
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| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 出版日期 | 2010 |
| 卷号 | 285期号:53页码:41982-41992 |
| 通讯作者邮箱 | mlong@imech.ac.cn ; zstressc@online.sh.cn |
| ISSN号 | 0021-9258 |
| 产权排序 | [Su, Weiai] Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China; [Lue, Shouqin; Long, Mian] Chinese Acad Sci, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China; [Lue, Shouqin; Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Inst Mech, Beijing 100190, Peoples R China |
| 通讯作者 | Long, MA (reprint author), 15 N 4th Ring Rd, Beijing 100190, Peoples R China. |
| 中文摘要 | Molecular selection, ion exclusion, and water permeation are well known regulatory mechanisms in aquaporin. Water permeability was found to be diverse in different subgroups of plasma membrane intrinsic proteins (PIPs), even though the residues surrounding the water holes remained the same across the subgroups. Upon homology modeling and structural comparison, a conserved Ala/Ile(Val) residue difference was identified in helix 2 that affected the conformation of the NPA region and consequently influenced the water permeability. The residue difference was found to be conservative within the two subgroups of PIPs in rice as well as in other plants. Functional tests further confirmed the prediction via site-directed mutagenesis where replacement of Ala(103) or Ala(102) in respective OsPIP1; 1 or OsPIP1; 3 with Val yielded 7.0- and 2.2-fold increases in water transportation, and substitution of Ile(98) or Val(95) in respective OsPIP2; 3 or OsPIP2; 7 with Ala resulted in 73 or 52% reduction of water transportation. Based on structural analyses and molecular dynamics simulations, we proposed that the difference in water permeability was attributed to the orientation variations of helix 2 that modified water-water and water-protein interactions. |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MOLECULAR-DYNAMICS ; AQUAPORIN NTAQP1 ; CHANNEL ACTIVITY ; STRUCTURAL BASIS ; TRANSPORT ; FAMILY ; ARABIDOPSIS ; SELECTIVITY ; PERMEATION ; GLYCEROL |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285622600084 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/58592]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Zhang MH,Lv SQ,Li GW,et al. Identification of A Residue in Helix 2 of Rice Plasma Membrane Intrinsic Proteins That Influences Water Permeability[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2010,285(53):41982-41992. |
| APA | Zhang MH.,吕守芹.,Li GW.,Mao ZL.,Yu X.,...&Su WA.(2010).Identification of A Residue in Helix 2 of Rice Plasma Membrane Intrinsic Proteins That Influences Water Permeability.JOURNAL OF BIOLOGICAL CHEMISTRY,285(53),41982-41992. |
| MLA | Zhang MH,et al."Identification of A Residue in Helix 2 of Rice Plasma Membrane Intrinsic Proteins That Influences Water Permeability".JOURNAL OF BIOLOGICAL CHEMISTRY 285.53(2010):41982-41992. |
入库方式： OAI收割
来源：力学研究所
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