Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing
文献类型：期刊论文
| 作者 | Wang, Zhaojun1; Lei, Ming1 ; Yao, Baoli1; Cai, Yanan1; Liang, Yansheng1; Yang, Yanlong1; Yang, Xibin2; Li, Hui2; Xiong, Daxi2
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| 刊名 | biomedical optics express
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 6期号:11页码:4353-4364 |
| ISSN号 | 2156-7085 |
| 产权排序 | 1 |
| 英文摘要 | autofocusing is a routine technique in redressing focus drift that occurs in time-lapse microscopic image acquisition. to date, most automatic microscopes are designed on the distance detection scheme to fulfill the autofocusing operation, which may suffer from the low contrast of the reflected signal due to the refractive index mismatch at the water/glass interface. to achieve high autofocusing speed with minimal motion artifacts, we developed a compact multi-band fluorescent microscope with an electrically tunable lens (etl) device for autofocusing. a modified searching algorithm based on equidistant scanning and curve fitting is proposed, which no longer requires a single-peak focus curve and then efficiently restrains the impact of external disturbance. this technique enables us to achieve an autofocusing time of down to 170 ms and the reproductivity of over 97%. the imaging head of the microscope has dimensions of 12 cm x 12 cm x 6 cm. this portable instrument can easily fit inside standard incubators for real-time imaging of living specimens. (c) 2015 optical society of america |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | biochemical research methods ; optics ; radiology, nuclear medicine & medical imaging |
| 类目[WOS] | biochemical research methods ; optics ; radiology, nuclear medicine & medical imaging |
| 研究领域[WOS] | biochemistry & molecular biology ; optics ; radiology, nuclear medicine & medical imaging |
| 关键词[WOS] | liquid-crystal ; focus |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000366122400015 |
| 源URL | [http://ir.opt.ac.cn/handle/181661/27661]  |
| 专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China 2.Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Jiangsu Key Lab Med Opt, Suzhou 215163, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhaojun,Lei, Ming,Yao, Baoli,et al. Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing[J]. biomedical optics express,2015,6(11):4353-4364. |
| APA | Wang, Zhaojun.,Lei, Ming.,Yao, Baoli.,Cai, Yanan.,Liang, Yansheng.,...&Xiong, Daxi.(2015).Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing.biomedical optics express,6(11),4353-4364. |
| MLA | Wang, Zhaojun,et al."Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing".biomedical optics express 6.11(2015):4353-4364. |
入库方式： OAI收割
来源：西安光学精密机械研究所
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