Cathepsin S of Sciaenops ocellatus: Identification, transcriptional expression and enzymatic activity
文献类型：期刊论文
| 作者 | Sun, Bo-guang1,2 ; Chi, Heng1,2
|
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2016 |
| 卷号 | 82期号:2016页码:76-82 |
| 关键词 | Cathepsin S Sciaenops ocellatus Teleost fish |
| 英文摘要 | Cathepsin S is a member of cysteine cathepsins and belongs to the cathepsin L-like family. In mammals, it is known to participate in various physiological processes and host immune defense. In teleost fish, the function of cathepsin S is less investigated. In the present work, we characterized a cathepsin S homologue (SoCatS) from red drum (Sciaenops ocellatus), a commercially valuable fish in Chinese mariculture. Like all cathepsin S, SoCatS possesses a peptidase domain with four catalytically essential residues (Gln140, Cys146, His285, and Asn305) conserved in the cathepsin S of different organisms. SoCatS shares 60-90% overall sequence identities with known teleost cathepsin S. Phylogenetic profiling indicated that SoCatS is evolutionally close to the cathepsin S of other teleost fish, especially Miichthys miiuy, a member of Sciaenidae family like red drum. SoCatS expression was detected in various tissues and was enhanced by bacterial infection. Purified recombinant SoCatS exhibited apparent peptidase activity with maximum at 50 degrees C and pH 7.5. This activity depended on the catalytic residue Cys146 and was severely reduced by the cathepsin inhibitor E-64. Our results suggest that SoCatS functions as a cysteine protease which is probably involved in the antibacterial immunity of red drum. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000367425100011 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/86650]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Bo-guang,Chi, Heng. Cathepsin S of Sciaenops ocellatus: Identification, transcriptional expression and enzymatic activity[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,82(2016):76-82. |
| APA | Sun, Bo-guang,&Chi, Heng.(2016).Cathepsin S of Sciaenops ocellatus: Identification, transcriptional expression and enzymatic activity.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,82(2016),76-82. |
| MLA | Sun, Bo-guang,et al."Cathepsin S of Sciaenops ocellatus: Identification, transcriptional expression and enzymatic activity".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 82.2016(2016):76-82. |
入库方式： OAI收割
来源：海洋研究所
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