Signal amplification of glucosamine-6-phosphate based on ribozyme glmS
文献类型：期刊论文
| 作者 | Zhao, Yongyun ; Chen, Haodong ; Du, Feng ; Yasmeen, Afshan ; Dong, Juan ; Cui, Xin ; Tang, Zhuo |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2014 |
| 卷号 | 62页码:337-342 |
| 关键词 | glmS riboswitch Glucosamine-6-phosphate Colorimetric assay Isothermal amplification Strand-displacement amplifications |
| 产权排序 | 1 |
| 通讯作者 | Cui, Xin ; Tang, Zhuo |
| 合作状况 | 其它 |
| 英文摘要 | Ribozyme glmS based isothermal amplification assay is developed for the colorimetric detection of glucosamine-6-phosphate (GlcN6P). Upon binding to the metabolite target GlcN6P, self-cleavage of glmS ribozyme is initiated to release RNA fragment that can trigger the cascade signal amplification to release large amount of G-quadruplex DNAzymes as reporter for colorimetric detection. Given the importance of GlcN6P for cell wall biosynthesis, the glmS riboswitch has become a new drug target for the development of antibiotics. This assay not only offers a convenient detection of GlcN6P with high specificity and sensitivity, but also provides a platform for high-throughput screening of antibiotics based on glmS riboswitches. (C) 2014 Elsevier B.V. All rights reserved. |
| 学科主题 | Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://210.75.237.14/handle/351003/26654]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhao, Yongyun,Chen, Haodong,Du, Feng,et al. Signal amplification of glucosamine-6-phosphate based on ribozyme glmS[J]. BIOSENSORS & BIOELECTRONICS,2014,62:337-342. |
| APA | Zhao, Yongyun.,Chen, Haodong.,Du, Feng.,Yasmeen, Afshan.,Dong, Juan.,...&Tang, Zhuo.(2014).Signal amplification of glucosamine-6-phosphate based on ribozyme glmS.BIOSENSORS & BIOELECTRONICS,62,337-342. |
| MLA | Zhao, Yongyun,et al."Signal amplification of glucosamine-6-phosphate based on ribozyme glmS".BIOSENSORS & BIOELECTRONICS 62(2014):337-342. |
入库方式： OAI收割
来源：成都生物研究所
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