Lipase Ligands in Nelumbo nucifera Leaves and Study of Their Binding Mechanism
文献类型：期刊论文
| 作者 | Zhu, Yuan-Ting ; Jia, Yan-Wei ; Liu, Yi-Ming ; Liang, Jian ; Ding, Li-Sheng ; Liao, Xun |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2014 |
| 卷号 | 62期号:44页码:10679-10686 |
| 关键词 | Nelumbo nucifera leaves ligand fishing lipase inhibitors fluorescence quenching molecular docking |
| 产权排序 | 1 |
| 通讯作者 | Liao, X (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China. |
| 合作状况 | 其它 |
| 英文摘要 | Lotus (Nelumbo nucifera) leaves have been widely used in weight-loss foods to prevent obesity in China. In this work, a facile procedure based on ligand fishing was developed to isolate and identify lipase inhibitors present in lotus leaves. Highly stable and active lipase-Fe3O4 superparamagnetic nanoparticle conjugates (LMNPs) were prepared and used as baits. Two flavonoids in lotus leaf extract were found to bind to the baits and were identified as quercetin-3-O-beta-d-arabinopyranosyl-(1?2)- beta-d-galactopyranoside (1) and quercetin-3-O-beta-d-glucuronide (4) based on electrospray ionization-mass spectrometric analyses. Their 50% inhibitory concentrations on lipase (IC50) were 52.9 +/- 3.2 and 17.1 +/- 1.5 mu g/mL, respectively. In addition, they were found to significantly quench the fluorescence of lipase, suggesting their strong affinities with this enzyme, which was further evidenced by molecular docking. Ligand fishing based on LMNPs shows great power for fast screening and identification of lipase inhibitors present in edible and medicinal plants. |
| 学科主题 | Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://210.75.237.14/handle/351003/26661]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Zhu, Yuan-Ting,Jia, Yan-Wei,Liu, Yi-Ming,et al. Lipase Ligands in Nelumbo nucifera Leaves and Study of Their Binding Mechanism[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(44):10679-10686. |
| APA | Zhu, Yuan-Ting,Jia, Yan-Wei,Liu, Yi-Ming,Liang, Jian,Ding, Li-Sheng,&Liao, Xun.(2014).Lipase Ligands in Nelumbo nucifera Leaves and Study of Their Binding Mechanism.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(44),10679-10686. |
| MLA | Zhu, Yuan-Ting,et al."Lipase Ligands in Nelumbo nucifera Leaves and Study of Their Binding Mechanism".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.44(2014):10679-10686. |
入库方式： OAI收割
来源：成都生物研究所
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