A novel colorimetric PCR-based biosensor for detection and quantification of hepatitis B virus
文献类型：期刊论文
| 作者 | Yang, Li ; Du, Feng ; Chen, Gangyi ; Yasmeen, Afshan ; Tang, Zhuo |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2014 |
| 卷号 | 840期号:2014页码:75-81 |
| 关键词 | Hepatitis B virus molecular diagnosis Biosensor G-quadruplex DNAzyme Peroxidase-like activity Colorimetric PCR |
| 产权排序 | 1 |
| 通讯作者 | Tang, Z (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Peoples R China. |
| 合作状况 | 其它 |
| 英文摘要 | Hepatitis B virus (HBV) can cause viral infection that attacks the liver and it is a major global health problem that put people at a high risk of death from cirrhosis of the liver and liver cancer. HBV has infected one third of the worldwide population, and 350 million people suffer from chronic HBV infection. For these reasons, development of an accurate, sensitive and expedient detection method for diagnosing, monitoring and assessing therapeutic response of HBV is very necessary and urgent for public health and disease control. Here we report a new strategy for detection of viral load quantitation of HBV based on colorimetric polymerase chain reaction (PCR) with DNAzyme-containing probe. The special DNAzyme adopting a G-quadruplex structure exhibited peroxidase-like activity in the presence of hemin to report colorimetric signal. This method has shown a broad range of linearity and high sensitivity. This study builds important foundation to achieve the specific and accurate detection level of HBV DNA with a low-cost and effective method in helping diagnosing, preventing and protecting human health form HBV generally all over the world and especially in developing countries. (C) 2014 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry, Analytical |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-02-26 |
| 源URL | [http://210.75.237.14/handle/351003/26670]  |
| 专题 | 成都生物研究所_天然产物研究 |
| 推荐引用方式 GB/T 7714 | Yang, Li,Du, Feng,Chen, Gangyi,et al. A novel colorimetric PCR-based biosensor for detection and quantification of hepatitis B virus[J]. ANALYTICA CHIMICA ACTA,2014,840(2014):75-81. |
| APA | Yang, Li,Du, Feng,Chen, Gangyi,Yasmeen, Afshan,&Tang, Zhuo.(2014).A novel colorimetric PCR-based biosensor for detection and quantification of hepatitis B virus.ANALYTICA CHIMICA ACTA,840(2014),75-81. |
| MLA | Yang, Li,et al."A novel colorimetric PCR-based biosensor for detection and quantification of hepatitis B virus".ANALYTICA CHIMICA ACTA 840.2014(2014):75-81. |
入库方式： OAI收割
来源：成都生物研究所
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